Next-Generation Sequencing Reveals Four Novel Viruses Associated with Calf Diarrhea

文献类型： 外文期刊

第一作者： Wu, Qi

作者： Wu, Qi;Li, Jizong;Wang, Wei;Zhou, Jinzhu;Wang, Dandan;Fan, Baochao;Zhang, Xuehan;Li, Bin;Wu, Qi;Gong, Ga;Suolang, Sizhu;Li, Jizong;Wang, Wei;Zhou, Jinzhu;Wang, Dandan;Fan, Baochao;Zhang, Xuehan;Li, Bin;Li, Jizong;Wang, Wei;Zhou, Jinzhu;Wang, Dandan;Li, Bin;Li, Jizong;Wang, Wei;Zhou, Jinzhu;Wang, Dandan;Li, Bin;Sun, Dongbo

作者机构：

关键词： next-generation sequencing; genome analysis; calf diarrhea; bovine kobuvirus; bovine norovirus; bovine astrovirus; bovine coronavirus

期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）

ISSN：

年卷期： 2021 年 13 卷 10 期

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收录情况： SCI

摘要： Calf diarrhea is one of the common diseases involved in the process of calf feeding. In this study, a sample of calf diarrhea that tested positive for bovine coronavirus and bovine astrovirus was subjected to high-throughput sequencing. The reassembly revealed the complete genomes of bovine norovirus, bovine astrovirus, bovine kobuvirus, and the S gene of bovine coronavirus. Phylogenetic analysis showed that the ORF2 region of bovine astrovirus had the lowest similarity with other strains and gathered in the Mamastrovirus unclassified genogroup, suggesting a new serotype/genotype could appear. Compared with the most closely related strain, there are six amino acid mutation sites in the S gene of bovine coronavirus, most of which are located in the S1 subunit region. The bovine norovirus identified in our study was BNoV-GIII 2, based on the VP1 sequences. The bovine kobuvirus is distributed in the Aichi virus B genus; the P1 gene shows as highly variable, while the 3D gene is highly conserved. These findings enriched our knowledge of the viruses in the role of calf diarrhea, and help to develop an effective strategy for disease prevention and control.

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