Recombinant nanobody expressed by transgenic chimeric chickens eliminated vertical transmission of avian leukosis virus subgroup J

文献类型： 外文期刊

第一作者： Liang, Jiahua

作者： Liang, Jiahua;Qin, Ke;Zhao, Yingying;Han, Ruiying;Wang, Jinhan;Liu, Jiahan;Lu, Huicheng;Wang, Xiaoli;Tang, Xiaochuan;Xiao, Ning;Yu, Fuxian

作者机构：

关键词： Avian leukosis virus subgroup J; Nanobody; Adenovirus; Transgenic chimeric chickens; Vertical transmission

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：2.7； 五年影响因子：2.9 ）

ISSN： 0378-1135

年卷期： 2025 年 302 卷

页码：

收录情况： SCI

摘要： Because of the vertical transmission of avian leukosis virus subgroup J (ALV-J), control of ALV-J in breed of chicken is still a serious issue. Blocking vertical transmission using antibodies is a potential strategy, but its high cost limits its application. We artificially designed recombinant nanobody (Nb) and efficiently expressed and secreted them in three primary chicken cells cultured in vitro by adenovirus delivery. We found that secreted the Nb could reduce the infection of cells by ALV-J. Moreover, we prepared transgenic chimeric chickens expressing Nb and found that the Nb reduced the infection and vertical transmission of ALV-J to chickens. In particular, transgenic chimeric hens expressing high concentration of the Nb eliminated vertical transmission of the virus. Collectively, current study provides new avenues for the control of ALV-J transmission.

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