CRISPR/Cas9-mediated knockout of Bsr-d1 enhances the blast resistance of rice in Northeast China

文献类型： 外文期刊

第一作者： Zhang, Ying

作者： Zhang, Ying;Lin, Xiu-Feng;Song, Anqi;Gao, Mengmeng;Jin, Yong-Mei;Zhang, Ying;Piao, Ri-Hua;Li, Li;Wu, Songquan;Song, Anqi;Gao, Mengmeng

作者机构：

关键词： Bsr-d1; Rice blast; Japonica rice; CRISPR/Cas9

期刊名称：PLANT CELL REPORTS （ 影响因子：6.2； 五年影响因子：5.9 ）

ISSN： 0721-7714

年卷期： 2024 年 43 卷 4 期

页码：

收录情况： SCI

摘要： Rice blast is a global disease that has a significant negative impact on rice yield and quality. Due to the complexity and variability of the physiological races of rice blast, controlling rice blast is challenging in agricultural production. Bsr-d1, a negative transcription factor that confers broad-spectrum resistance to rice blast, was identified in the indica rice cultivar Digu; however, its biological function in japonica rice varieties is still unclear. In this study, we analyzed the blast resistance allele of Bsr-d1 in a total of 256 japonica rice varieties from Jilin Province in Northeast China and found that this allele was not present in these varieties. Therefore, we generated Bsr-d1 knockout mutants via the CRISPR/Cas9 system using the japonica rice variety Jigeng88 (JG88) as a recipient variety. Compared with those of the wild-type JG88, the homozygous Bsr-d1 mutant lines KO#1 and KO#2 showed enhanced leaf blast resistance at the seedling stage to several Magnaporthe oryzae (M. oryzae) races collected from Jilin Province in Northeast China. Physiological and biochemical indices revealed that the homozygous mutant lines produced more hydrogen peroxide than did JG88 plants when infected with M. oryzae. Comparative RNA-seq revealed that the DEGs were mainly involved in the synthesis of amide compounds, zinc finger proteins, transmembrane transporters, etc. In summary, our results indicate that the development of Bsr-d1 knockout mutants through CRISPR/Cas9 can enhance the broad-spectrum resistance of rice in Northeast China to rice blast. This study not only provides a theoretical basis for disease resistance breeding involving the Bsr-d1 gene in Northeast China, but also provides new germplasm resources for disease-resistance rice breeding.

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