Molecular cloning, genomic structure and expression analysis of major histocompatibility complex class I alpha gene of half-smooth tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

第一作者： Chen, Song-lin

作者： Chen, Song-lin;Xu, Tian-jun

作者机构：

关键词： Half-smooth tongue sole;MHC I alpha;Cloning;Sequence analysis;Gene expression profile

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN： 0920-1742

年卷期： 2011 年 37 卷 1 期

页码：

收录情况： SCI

摘要： Major histocompatibility complex (MHC) has a central role in the adaptive immune system by presenting foreign peptide to the T-cell receptor. The full length of MHC class I alpha cDNA was cloned from half-smooth tongue sole by homology cloning and rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR), genomic organization and expression of MHC I alpha were examined to study the function of MHC gene in fish. The domain structure feature and antigen-binding motifs of other teleost and mammals MHC are conserved in the half-smooth tongue sole MHC I alpha gene. The deduced amino acid sequence of half-smooth tongue sole MHC I alpha (GenBank accession no. FJ372720) had 12.1-61.8% identity with those of human and other fish. Eight exons and seven introns were identified in MHC I alpha gene. Real-time quantitative PCR demonstrated that MHC I alpha gene was ubiquitously expressed in normal tissues, while that in Vibrio anguillarum infected fish was significantly increased in intestines and decreased in spleen and liver from 24 to 72 h after infection, followed by a recovery to normal level after 96 h.

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