Differential detection of ovine Theileria species using loop-mediated isothermal amplification combined with nanoparticle-based lateral flow biosensor

文献类型： 外文期刊

第一作者： Zhu, Haohan

作者： Zhu, Haohan;Zhao, Shuaiyang;Luo, Jin;Obaid, Muhammad Kashif;Zhang, Shaohua;Liu, Peiqi;Luo, Jianxun;Yin, Hong;Guan, Guiquan;Liu, Junlong;Yin, Hong

作者机构：

关键词： T. luwenshuni; T. uilenbergi; T. ovis; 28S rRNA; LAMP-LFB

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.2； 五年影响因子：2.4 ）

ISSN： 0304-4017

年卷期： 2025 年 336 卷

页码：

收录情况： SCI

摘要： Ovine theileriosis is an important tick-borne protozoan disease. It has been reported that three Theileria species are responsible for ovine theileriosis in China, which are T. luwenshuni, T. uilenbergi, and T. ovis. Here, we established three detection techniques based on loop-mediated isothermal amplification (LAMP) and nanoparticle-based lateral flow biosensor (LFB) for the infection of the three Theileria species. Three LAMP primer sets were designed targeting the nucleotide sequences of the 28S rRNA gene of T. luwenshuni, T. uilenbergi, and T. ovis. We used LAMP coupled with real-time fluorescence detection to optimize the concentrations of dNTP Mix, MgSO4, and Bst 2.0 DNA polymerase, as well as the reaction temperature of the LAMP assay, and then combined LAMP with LFB (LAMP-LFB). The entire detection assay process, including genomic DNA extraction (40 min), LAMP reaction (40 min), and LFB readout (<5 min), can be completed within 85 min. The established assays can specifically detect species of T. luwenshuni, T. uilenbergi, and T. ovis infection without cross-reaction with other Theileria, Babesia, and Anaplasma species. The detection limits of the LAMP-LFB assays for T. luwenshuni, T. uilenbergi, and T. ovis plasmid templates were 2.72 x 10(2) copies/mu L, 2.96 x 10(3) copies/mu L, and 3.05 x 10(1) copies/mu L, respectively. Finally, we compared the established LAMP-LFB assay with the traditional PCR assay. The results showed that the total coincidence rates were 96.67 % (T. luwenshuni), 96.67 % (T. uilenbergi), and 93.33 % (T. ovis), respectively. In general, we developed a rapid, simple, sensitive, and specific technique for differential detection of T. luwenshuni, T. uilenbergi, and T. ovis infection in small ruminants.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Epidemiology, molecular diversity and zoonotic implications of Echinococcus granulosus sensu stricto (G1) in cattle across Nigeria: Evidence of broader transmission dynamics

    作者：Amuda, Tharheer Oluwashola;Li, Li;Wang, Li-Qun;Dai, Guo-Dong;Chen, Wei-Gang;Wu, Yao-Dong;Wu, Ke-Ke;Wu, Yixuan;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong;Yan, Hong-Bin;Amuda, Tharheer Oluwashola;Li, Li;Wang, Li-Qun;Dai, Guo-Dong;Chen, Wei-Gang;Wu, Yao-Dong;Wu, Ke-Ke;Wu, Yixuan;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong;Yan, Hong-Bin;Morenikeji, Olajumoke Abimbola;Odaibo, Georgina Nijideka;Onoja, Bernard Anyebe;Akanbi, Ibikunle Mark;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong

    关键词：Echinococcus granulosus sensu lato; Cystic echinococcosis; Nigeria; Genetic diversity; Molecular epidemiology; One health

  • A new intermediate host of Echinococcus shiquicus in Qinghai-Tibet Plateau, China

    作者：Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Hou, Xue-Yi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Guo, Min-Kuan;Li, Wen-Dong;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Li, Wen-Dong;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Shumuye, Nigus Abebe;Gao, Sheng-Zhi;Hou, Xue-Yi;Zhonggecao, Tao;Zhaxilamao, Bao-Quan;Gazangzhuoma, Hong;Doukao, Hong-Bin;Zhang, Fu-Heng;Guo, Min-Kuan;Li, Wen-Dong;Yang, Tao;Fu, Bao-Quan;Yin, Hong;Jia, Wan-Zhong

    关键词：Echinococcus shiquicus; Plateau zokor; Intermediate host; Qinghai-Tibet Plateau; Genetic diversity

  • Empowering household energy transition: The impact of digital economy on clean energy use in rural China

    作者：Zhang, Shaohua;Sang, Xiance;Luo, Xiaofeng;Yang, Wei

    关键词：Digital economy; Household energy transition; Capital; Rural area; China

  • Epidemiology and Molecular Detection of Tick-Borne Rickettsiae in Hyalomma and Rhipicephalus Ticks Infesting Dogs and Sheep in Pakistan

    作者：Zeb, Jehan;Zeb, Jehan;Zeb, Jehan;Senbill, Haytham;Obaid, Muhammad Kashif;Ren, Qiaoyun;Said, Mourad Ben;Said, Mourad Ben;Aziz, Muhammad Umair;Sparagano, Olivier Andre;Khan, Adil;Alajmi, Reem;Cossio-Bayugar, Raquel;Dzul-Rosado, Karla

    关键词：co-infection; Hyalomma; prevalence; Rhipicephalus; rickettsia; tick-borne pathogens

  • Classification of different gluten wheat varieties based on hyperspectral preprocessing, feature screening, and machine learning

    作者：Qi, Xinghui;Zhang, Shaohua;Wang, Liyang;Zhang, Haiyan;Feng, Wei;Wang, Chenyang;Guo, Tiancai;He, Li;Zhang, Haiyan;Feng, Wei;Wang, Chenyang;Guo, Tiancai;He, Li;Hu, Xuexu

    关键词：Wheat; Gluten type; Feature selection; Preprocessing; Machine learning

  • Rapid visual detection of Moniezia spp. in sheep feces via Recombinase Polymerase Amplification-Lateral Flow Dipstick (RPA-LFD) assay

    作者：Zhang, Shaohua;Zhao, Yeping;Liang, Weijia;Wang, Shuai;Cui, Xiu;Zhu, Haohan;Zhang, Yueyue;Li, Huimin;Mu, Wenjie;Guo, Aijiang;Zhang, Shaohua;Zhao, Yeping;Cui, Xiu;Liu, Xiaolei;Li, Huimin;Liang, Weijia

    关键词：Monieziasis; Recombinase polymerase amplification (RPA); Lateral flow dipstick (LFD); Visual detection; beta-tubulin gene

  • Expanding the molecular understanding of Theileria infections in cattle and yaks from Gansu and Qinghai, China

    作者：Chai, Yijun;Che, Jin;Zhao, Shuaiyang;Wang, Jinming;Guan, Guiquan;Yin, Hong;Che, Jin;Yin, Hong

    关键词：Molecular prevalence; Theileriosis; Piroplasm; Bovine

微信小程序