The Structural Deciphering of the α3 Helix Within ZmHsfA2'S DNA-Binding Domain for the Recognition of Heat Shock Elements in Maize

文献类型： 外文期刊

第一作者： Wang, Yantao

作者： Wang, Yantao;Zhao, Min;Ma, Zhenyu;Li, Guoliang;Meng, Xiangzhao;Duan, Shuonan;Liu, Zihui;Guo, Xiulin;Zhang, Huaning

作者机构：

关键词： maize; heat shock transcription factor; heat shock element; arginine

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2025 年 14 卷 13 期

页码：

收录情况： SCI

摘要： Heat shock transcription factor (Hsf) plays a pivotal role in regulating plant growth, development, and stress responses. Hsf activates or represses target gene transcription by binding to the heat shock element (HSE) of downstream genes. However, the specific interaction sites between Hsf and the HSE in the promoter remain unclear. In this study, the critical amino acid residues of ZmHsf17 and the paralogous ZmHsf05 involved in DNA binding were identified using molecular docking models, site-directed mutagenesis, and the electrophoretic mobility shift assay (EMSA). The results reveal that both ZmHsf17 and ZmHsf05 bind to the HSE of the ZmPAH1 promoter via a conserved arginine residue located in the alpha 3 helix of their DNA-binding domains. Sequence substitution experiments among distinct HSEs demonstrated that flanking sequences upstream and downstream of the HSE core synergistically contribute to the specificity of DNA-binding domain recognition. Comparative evolutionary analysis of DNA-binding domain sequences from 25 phylogenetically diverse species reveals that the alpha 3 helix constitutes the most conserved structural element. This study elucidates the key interaction sites between maize HsfA2 and its target genes, providing theoretical insights into the binding specificity to the HSEs of the plant's Hsf family and the functional divergence. Additionally, these findings offer new targets for the precise engineering of Hsf proteins and synthetic HSEs.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Dynamic changes of quality characteristics during fermentation of orange-fleshed sweet potato alcoholic beverage

    作者：Wu, Huimin;Fu, Qinquan;Tang, Tiantian;Liu, Yinqi;Ma, Xinyi;Zhao, Min;Zhou, Xin;Wang, Bin;Shi, Xuewei;Yang, Yiling;Wang, Zhangying;Yang, Yiling;Wang, Zhangying;Wang, Bin;Shi, Xuewei;Wang, Bin;Shi, Xuewei;Wang, Bin;Shi, Xuewei

    关键词：Orange-fleshed sweet potato (OFSP); Alcoholic beverage; Antioxidant properties; Physicochemical indicators; Volatile compounds

  • Simultaneous profiling of chromatin-associated RNA at targeted DNA loci and RNA-RNA Interactions through TaDRIM-seq

    作者：Ding, Cheng;Chen, Guoting;Luan, Shiping;Gao, Runxin;Fan, Yudong;Zhang, Ying;Wang, Xiaoting;Li, Guoliang;Foda, Mohamed F.;Yan, Jiapei;Li, Xingwang;Chen, Guoting;Li, Guoliang;Chen, Guoting;Li, Guoliang;Foda, Mohamed F.;Li, Xingwang;Li, Xingwang

    关键词：

  • Degradable film mulching recruited beneficial microbiota and increased rhizosphere bacterial diversity in sunflower

    作者：Meng, Tiantian;Lu, Zhanyuan;Meng, Tiantian;Zhang, Xiangqian;Zhang, Jianwei;Lu, Zhanyuan;Zhao, Xiaoyu;Bu, Hengtong;Chen, Xuanyi;Zhao, Min;Zhang, Dejian;Lu, Zhanyuan;Wang, Weini;Liu, Junmei;Zhang, Xiangqian;Lu, Zhanyuan

    关键词：Degradation film; Sunflower; Root niche; Microbial community assembly; Yield

  • EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research

    作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang

    关键词：metagenome; microbiome; microbiota; pipeline; visualization

  • Molecular characterization and functional roles of NAC transcription factors in regulating chlorophyll degradation during litchi fruit ripening

    作者：Yang, Yabing;Zhang, Bo;Chen, Lei;Zhao, Min;Huang, Qiaoying;Wang, Miao;Zhang, Zhike;Qin, Yonghua;Zhao, Jietang;Hu, Guibing;Wang, Dan

    关键词：NAC transcription factor; Chlorophyll degradation; S GR; Litchi chinensis Sonn

  • A Novel Large-Particle Slow-Release Fertilizer Improves Nutrient Use Efficiency and Yield of Cassava by Boundary Layer Limitation

    作者：He, Cuicui;Wang, Hua;Huang, Jie;Wang, Dengfeng;Qin, Xindao;Zhang, Wen;Wu, Dongming;Jiu, Yuanda;Zhao, Min;Xie, Yi;Chen, Qingmian;Zhou, Rongfei;Li, Guichun;Xu, Minggang

    关键词：large-particle slow-release fertilizer; slow-release performance; agronomic efficiency; kinetic modeling; nutrient transport

  • Identification of GSK3 Family Genes in Pear and Their Expression Analysis Under Drought Stress

    作者：Hu, Kairan;Zhang, Ziyi;Zhao, Shuliang;Wang, Qingjiang;Cheng, Fuhou;Li, Guoliang;Zhang, Yali

    关键词：pear; GSK3; genome-wide; expression pattern

微信小程序