Development of Magnetic Lateral Flow and Direct Competitive Immunoassays for Sensitive and Specific Detection of Halosulfuron-Methyl Using a Novel Hapten and Monoclonal Antibody

文献类型： 外文期刊

第一作者： Ying, Ying

作者： Ying, Ying;Cui, Xueyan;Li, Hui;Pan, Lingyi;Luo, Ting;Cao, Zhen;Wang, Jing;Ying, Ying;Cui, Xueyan;Li, Hui;Pan, Lingyi;Luo, Ting;Cao, Zhen;Wang, Jing

作者机构：

关键词： halosulfuron-methyl; monoclonal antibody; magnetic lateral flow immunoassay

期刊名称：FOODS （ 影响因子：5.2； 五年影响因子：5.5 ）

ISSN：

年卷期： 2023 年 12 卷 14 期

页码：

收录情况： SCI

摘要： Halosulfuron-methyl (HM) is widely used for the removal of noxious weeds in corn, sugarcane, wheat, rice, and tomato fields. Despite its high efficiency and low toxicity, drift to nontarget crops and leaching of its metabolites to groundwater pose potential risks. Considering the instability of HM, the pyrazole sulfonamide of HM was used to generate a hapten and antigen to raise a high-quality monoclonal antibody (Mab, designated 1A91H11) against HM. A direct competitive immunoassay (dcELISA) using Mab 1A91H11 achieved a half-maximal inhibitory concentration (IC50) of 1.5 x 10(-3) mg/kg and a linear range of 0.7 x 10(-3) mg/kg-10.7 x 10(-3) mg/kg, which was 10 times more sensitive than a comparable indirect competitive ELISA (icELISA) and more simple to operate. A spiking recovery experiment performed in tomato and maize matrices with 0.01, 0.05, and 0.1 mg/kg HM had average recoveries within 78.9-87.9% and 103.0-107.4% and coefficients of variation from 1.1-6.8% and 2.7-6.4% in tomato and maize, respectively. In addition, a magnetic lateral flow immunoassay (MLFIA) was developed for quantitative detection of low concentrations of HM in paddy water. Compared with dcELISA, the MLFIA exhibited 3.3- to 50-fold higher sensitivity (IC50 0.21 x 10(-3) mg/kg). The average recovery and RSD of the developed MLFIA ranged from 81.5 to 92.5% and 5.4 to 9.7%. The results of this study demonstrated that the developed dcELISA and MLFIA are suitable for rapid detection of HM residues in tomato and maize matrices and paddy water, respectively, with acceptable accuracy and precision.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序