Exploring the potential role of EPSPS mutations for enhanced glyphosate resistance in Nicotiana tabacum

文献类型： 外文期刊

第一作者： Li, Bingjie

作者： Li, Bingjie;Chen, Chen;Cui, Mengmeng;Sun, Yuhe;Lv, Jing;Dai, Changbo;Li, Bingjie;Chen, Chen

作者机构：

关键词： glyphosate; herbicide resistance; EPSP synthase; tobacco; gene editing

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）

ISSN： 1664-462X

年卷期： 2025 年 16 卷

页码：

收录情况： SCI

摘要： Glyphosate is a widely used non-selective, broad-spectrum, systemic herbicide by interfering with the biosynthesis of aromatic amino acids. However, the emergence of glyphosate-resistant weeds has driven the need for enhanced herbicide resistance in crops. In this study, we engineered two mutant variants of the tobacco EPSPS gene through amino acid substitution (TIPS-NtEPSPS and P180S-NtEPSPS). These mutated EPSPS genes were overexpressed in tobacco under the control of CaMV35S promoters. Our results demonstrate that overexpression of TIPS-NtEPSPS significantly enhances glyphosate tolerance, allowing plants to withstand up to four times the recommended dose without compromising their fitness. This research highlights the potential of the TIPS-NtEPSPS mutant to improve herbicide resistance in tobacco, offering a viable approach for effective weed management.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序