BcWRKY1 confers Botrytis cinerea susceptibility via inhibiting JA biosynthesis

文献类型： 外文期刊

第一作者： Yuan, Shuilin

作者： Yuan, Shuilin;Wang, Yuan;Hu, Die;Xiao, Dong;Wang, Jianjun;Hou, Xilin;Li, Ying;Yuan, Shuilin;Hu, Die

作者机构：

期刊名称：PHYSIOLOGIA PLANTARUM （ 影响因子：5.4； 五年影响因子：5.5 ）

ISSN： 0031-9317

年卷期： 2024 年 176 卷 4 期

页码：

收录情况： SCI

摘要： WRKYs play important roles in plant stress resistance. However, the role of WRKYs in non-heading Chinese cabbage (Brassica campestris ssp. chinensis) against Botrytis cinerea (B. cinerea) remains poorly understood. Herein, the expression of BcWRKY1 was induced by B. cinerea. Further, the role of BcWRKY1 in B. cinerea infection was identified. Silencing of BcWRKY1 in non-heading Chinese cabbage enhanced plant resistance to B. cinerea. After B. cinerea inoculation, BcWRKY1-silencing plants exhibited lower reactive oxygen species (ROS) content, higher jasmonic acid (JA) content, and the expression level of JA biosynthesis genes, BcOPR3, BcLOX3-1 and BcLOX3-2 were upregulated. Overexpression of BcWRKY1 in Arabidopsis exhibited a complementary phenotype. By directly targeting W-boxes in the promoter of BcLOX3-2, BcWRKY1 inhibited the transcription of this gene. In addition, 13 candidate interacting proteins of BcWRKY1 were identified by yeast two-hybrid (Y2H) screening, and the interaction between BcWRKY1 and BcCaM6 weakened the inhibition of BcLOX3-2. In summary, our findings suggest that BcWRKY1 interacts with BcCaM6 to negatively regulate disease resistance.

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