A phage-displayed nanobody-based competitive immunoassay for the detection of African swine fever virus antibodies
文献类型： 外文期刊
第一作者： Chen, Wenting
作者： Chen, Wenting;Yang, Jifei;Niu, Qingli;Wang, Jinming;Liu, Yanghe;Li, Xuesai;Zhao, Yaru;Zhang, Zhonghui;Liu, Zhijie;Guan, Guiquan;Yin, Hong;Yin, Hong;Liu, Zhijie
作者机构：
关键词： African swine fever; Nanobody; Phage display; K205R protein; ELISA
期刊名称：VIROLOGY JOURNAL （ 影响因子：3.8； 五年影响因子：3.7 ）
ISSN：
年卷期： 2025 年 22 卷 1 期
页码：
收录情况： SCI
摘要： African swine fever (ASF) is a highly infectious and devastating disease that poses a significant threat to the global swine industry. The rapid spread of ASF and its ongoing pandemics continue to impact pig farming worldwide. The absence of an effective vaccine, coupled with the complexity of the African swine fever virus (ASFV), makes the control and eradication of ASF a formidable challenge. Nanobodies, derived from camelids, have emerged as promising alternatives to conventional monoclonal antibodies, offering distinct advantages in various biological applications. In this study, specific nanobodies targeting the ASFV K205R protein were selected from a phage-displayed immune library. Ten individual nanobodies were isolated based on their complementary determining regions (CDRs), and four were found to bind to the naive K205R protein of ASFV. After evaluation, nanobody VHH1 was selected for the development of a competitive enzyme-linked immunosorbent assay (ELISA) for ASFV antibody detection. The assay was optimized for various reaction conditions, and the cut-off value was determined to be 26.85%, with diagnostic sensitivity and specificity of 97.52% and 97.48%, respectively. No cross-reactivity was observed with sera from pigs infected with other swine viruses, and the assay exhibited a detection sensitivity of 1:128. Comparative analysis of clinical samples showed a high concordance rate (98.98%) between the nanobody-based and monoclonal antibody-based ELISAs (Mab-cELISA). In conclusion, this study presents a phage-displayed nanobody-based competitive ELISA for the detection of ASFV antibodies, which could be valuable for ASF sero-surveillance. Additionally, the K205R-specific nanobodies identified here may be adapted for other biological or biomedical applications.
分类号：
- 相关文献
作者其他论文 更多>>
-
Overexpression of Cymbidium goeringii Cgo-miR159 Regulates Anther Dehiscence and Pollen Development in Arabidopsis and Tobacco
作者：Xu, Zihan;Shen, Jianshuang;Xu, Zihan;Liu, Qian;Hu, Fengrong;Chen, Yue;Wang, Jinming
关键词：miR159;
Cymbidium ; anther dehiscence; pollen development -
Metagenomic Investigation of Pathogenic RNA Viruses Causing Diarrhea in Sika Deer Fawns
作者：Wang, Weiyang;Wang, Qilin;Cao, Runlai;Li, Yacong;Liu, Ziyu;Xue, Zhuqing;Wang, Xiaoxu;Liu, Zhijie
关键词：sika deer; viral metagenomics; whole-genome sequencing; rotavirus
-
Epidemiology, molecular diversity and zoonotic implications of Echinococcus granulosus sensu stricto (G1) in cattle across Nigeria: Evidence of broader transmission dynamics
作者：Amuda, Tharheer Oluwashola;Li, Li;Wang, Li-Qun;Dai, Guo-Dong;Chen, Wei-Gang;Wu, Yao-Dong;Wu, Ke-Ke;Wu, Yixuan;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong;Yan, Hong-Bin;Amuda, Tharheer Oluwashola;Li, Li;Wang, Li-Qun;Dai, Guo-Dong;Chen, Wei-Gang;Wu, Yao-Dong;Wu, Ke-Ke;Wu, Yixuan;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong;Yan, Hong-Bin;Morenikeji, Olajumoke Abimbola;Odaibo, Georgina Nijideka;Onoja, Bernard Anyebe;Akanbi, Ibikunle Mark;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong
关键词：Echinococcus granulosus sensu lato; Cystic echinococcosis; Nigeria; Genetic diversity; Molecular epidemiology; One health
-
A new intermediate host of Echinococcus shiquicus in Qinghai-Tibet Plateau, China
作者：Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Hou, Xue-Yi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Guo, Min-Kuan;Li, Wen-Dong;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Li, Wen-Dong;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Shumuye, Nigus Abebe;Gao, Sheng-Zhi;Hou, Xue-Yi;Zhonggecao, Tao;Zhaxilamao, Bao-Quan;Gazangzhuoma, Hong;Doukao, Hong-Bin;Zhang, Fu-Heng;Guo, Min-Kuan;Li, Wen-Dong;Yang, Tao;Fu, Bao-Quan;Yin, Hong;Jia, Wan-Zhong
关键词：Echinococcus shiquicus; Plateau zokor; Intermediate host; Qinghai-Tibet Plateau; Genetic diversity
-
Antibody Responses and the Vaccine Efficacy of Recombinant Glycosyltransferase and Nicastrin Against Schistosoma japonicum
作者：Dong, Bowen;Zhong, Haoran;Zhu, Danlin;Li, Hao;Jin, Yamei;Wu, Luobin;Wang, Jinming;Wang, Jinming
关键词：
Schistosoma japonicum ; SjGT; SjNCSTN; immune protection -
Expanding the molecular understanding of Theileria infections in cattle and yaks from Gansu and Qinghai, China
作者：Chai, Yijun;Che, Jin;Zhao, Shuaiyang;Wang, Jinming;Guan, Guiquan;Yin, Hong;Che, Jin;Yin, Hong
关键词：Molecular prevalence; Theileriosis; Piroplasm; Bovine
-
Lacticaseibacillus rhamnosus G7 alleviates DSS-induced ulcerative colitis by regulating the intestinal microbiota
作者：Lao, Jianlong;Chen, Man;Yan, Shuping;Gong, Han;Wen, Zhaohai;Yong, Yanhong;Jia, Dan;Lv, Shuting;Zou, Wenli;Li, Junmei;Tan, Huiming;Liu, Zengyuan;Guo, Fucheng;Ju, Xianghong;Li, Youquan;Lao, Jianlong;Ju, Xianghong;Li, Youquan;Jia, Dan;Yin, Hong;Kong, Xiangying
关键词：Probiotics; Inflammatory;
Lacticaseibacillus rhamnosus G7; Gut microbiota
