Intelligent point-of-care test via smartphone-enabled microarray for multiple targets: Mycotoxins in food

文献类型： 外文期刊

第一作者： Wu, Wenqin

作者： Wu, Wenqin;Zhou, Dan;Chen, Xiaomei;Tang, Xiaoqian;Jiang, Jun;Yu, Li;Li, Hui;Zhang, Qi;Zhang, Zhaowei;Li, Peiwu;Wu, Wenqin;Yu, Li;Li, Hui;Zhang, Qi;Zhang, Zhaowei;Li, Peiwu;Wu, Wenqin;Zhang, Qi;Zhang, Zhaowei;Li, Peiwu;Wu, Wenqin;Chen, Xiaomei;Tang, Xiaoqian;Jiang, Jun;Zhang, Qi;Zhang, Zhaowei;Li, Peiwu;Wu, Wenqin;Zhang, Qi;Zhang, Zhaowei;Li, Peiwu

作者机构：

关键词： Simultaneous determination of mycotoxins; Portable microplate reader; Microarray; Smartphone-based readout; Food safety

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：8.4； 五年影响因子：7.2 ）

ISSN：

年卷期： 2022 年 360 卷

页码：

收录情况： SCI

摘要： The growing co-occurrence of multiple hazardous contaminations causes a notably increased risk to humans due to their synergistic and additive toxicological effects. Current methods rarely achieve on-site intelligent multiple targets detection, requesting a smartphone-based point-of-care test. In this study, an on-site sensitive smartphone-based microarray (SPM) via an indirect competitive immunoassay was developed for simultaneously detecting ochratoxin A (OTA), deoxynivalenol (DON), fumonisin B-1 (FB1), and zearalenone (ZEN) down to 30 min. The immunoassay signals generated by peroxidase and tetramethylbenzidine were recorded by a complementary metal-oxide-semiconductor transistor camera and analyzed via an Android App. Low limits of detection were found to be 6, 14, 66, and 5 pg/mL along with linear ranges of 0.08-4860, 0.09-3240, 0.09-3240, and 0.05-3240 ng/mL for OTA, DON, FB1, and ZEN, respectively. And a simple on-site sample preparation was developed to extract four mycotoxins in one single run. Validation results demonstrated high consistency between this SPM and HPLC-MS/MS method using real samples. This SPM can be extensively applied to environmental monitoring, food safety, and healthcare by altering antibodies and antigens.

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