Polymorphism and Balancing Selection of MHC Class II DAB Gene in 7 Selective Flounder (Paralichthys olivaceus) Families

文献类型： 外文期刊

第一作者： Du, Min

作者： Du, Min;Chen, Song-lin;Liang, You;Wang, Lei;Gao, Feng-tao;Liu, Yang;Liao, Xiao-Lin;Du, Min;Du, Min

作者机构：

期刊名称：EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE （ 影响因子：2.629； 五年影响因子：2.845 ）

ISSN： 1741-427X

年卷期： 2011 年

页码：

收录情况： SCI

摘要： In order to determine the genetic variation of the MHC class IIB exon2 allele in the offspring, 700 fry from seven families of Japanese flounder challenged with V. anguillarum were studied, and different mortality rates were found in those families. Five to ten surviving and dead fry from each of the seven families were selected to study the MHC class II B exon2 gene with PCR and a direct sequencing method. One hundred and sixteen different exon2 sequences were found and 116 different alleles were identified, while a minimum of four loci were revealed in the MHC class II B exon2 gene. The ratio (d(N)/d(S)) of nonsynonymous substitution (d(N)) to synonymous substitutions (d(S)) in the peptide-binding region (PBR) of the MHC class IIB gene was 6.234, which indicated that balancing selection is acting on the MHC class IIB genes. The MHC IIB alleles were thus being passed on to their progeny. Some alleles were significantly more frequent in surviving than dead individuals. All together our data suggested that the alleles Paol-DAB*4301, Paol-DAB*4601, Paol-DAB*4302, Paol-DAB*3803, and Paol-DAB*4101 were associated with resistance to V. anguillarum in flounder.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序