Chromosome-level genome assembly of stem borer Batocera rufomaculata using PacBio HiFi and Hi-C sequencing

文献类型： 外文期刊

第一作者： He, Jinwu

作者： He, Jinwu;Wan, Wenting;Dong, Zhiwei;Wang, Yangjie;Li, Xueyan;Bai, Tianqi;Bai, Tianqi;Zhang, Hongrui;Wang, Yangjie;Li, Xueyan;Li, Xueyan

作者机构：

期刊名称：SCIENTIFIC DATA （ 影响因子：6.9； 五年影响因子：8.7 ）

ISSN：

年卷期： 2025 年 12 卷 1 期

页码：

收录情况： SCI

摘要： Batocera rufomaculata (Cerambycidae: Lamiinae), a prominent representative of longhorned beetles, is a globally significant stem-boring pest, infesting over 50 species of deciduous trees. Despite its substantial ecological and economic impact, the genomic basis underlying its host adaptation remain poorly understood. Here, we present a chromosome-level genome assembly of B. rufomaculata, constructed using a combination of Illumina, PacBio HiFi, and Hi-C sequencing data. The genome spans 338.08 Mb, with a scaffold N50 of 37.00 Mb, and is organized into 10 pseudo-chromosomes, including a chromosome X validated by genome collinearity and sequencing depth analyses. Repetitive elements constitute 27.89% of the genome, totaling 94.29 Mb. Out of 17,887 predicted genes, 12,729 were functionally annotated with at least one supporting evidence. The high-quality genome assembly and annotation were confirmed by multiple metrics, including genome size, reads mapping rate (>99.5%), BUSCO completeness (>97.1%), and collinearity analyses. This comprehensive genomic resource provides a foundation for investigating the ecological adaptation of B. rufomaculata and offers valuable insights into the genetic mechanisms that could inform pest management strategies.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序