Characterization of the Glutamate Decarboxylase (GAD) Gene and Functional Analysis of DlGAD3 in the Accumulation of γ-Aminobutyric Acid in Longan (Dimocarpus longan Lour.) Pulp

文献类型： 外文期刊

第一作者： Wei, Weilin

作者： Wei, Weilin;Zhang, Tingting;Chen, Yongping;Zhou, Ziqi;Su, Wenbing;Xu, Qizhi;Zhang, Yaling;Zheng, Shaoquan;Jiang, Jimou;Deng, Chaojun

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关键词： longan; gamma-aminobutyric acid (GABA); glutamate decarboxylase (GAD); pulp; GABA transaminase (GABA-T)

期刊名称：HORTICULTURAE （ 影响因子：3.0； 五年影响因子：3.2 ）

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年卷期： 2025 年 11 卷 6 期

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收录情况： SCI

摘要： gamma-aminobutyric acid (GABA) is a four-carbon non-protein amino acid, with many regulatory effects in humans. It aids in regulating blood glucose levels and pressure and is widely recognized for its ability to promote cognitive balance through the alleviation of stress and improvements in sleep quality. The GABA content of longan pulp is higher than that of many other fruits and vegetables; however, much is still unknown about GABA's biosynthesis in longan. In this study, we found that the GABA content of 'Baoshi No. 1' (BS1) pulp was significantly higher than that of 'Chunxiang' (CX) pulp. The GAD activity was higher in BS1 pulp than CX pulp, while there was no significant difference in the GABA-T activity. Additionally, five GAD genes were identified in longan, and an analysis of their transcriptional levels showed that only the expression level of DlGAD3 corresponded to the GABA content and GAD activity. DlGAD3 was localized in the cytoplasm, and its transient overexpression promoted an increase in the GABA content in Nicotiana benthamiana leaves. Overall, our results show that DlGAD3 is able to promote the accumulation of GABA and may play a major role in its biosynthesis in longan pulp.

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