Overexpression of SQUALENE SYNTHASE Reduces Nicotiana benthamiana Resistance against Phytophthora infestans

文献类型： 外文期刊

第一作者： Fu, Ke-Ke

作者： Fu, Ke-Ke;Feng, Hongjie;Fu, Ke-Ke;Wan, Wei;Cai, Yanling;Zhou, Shaoqun;Liang, Junhao;Jing, Xiangfeng;Wan, Wei

作者机构：

关键词： potato late blight; tomato late blight quantitative disease resistance; triterpenoids; phytosterols

期刊名称：METABOLITES （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN：

年卷期： 2023 年 13 卷 2 期

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收录情况： SCI

摘要： Plant triterpenoids play a critical role in plant resistance against Phytophthora infestans de Bary, the causal pathogen of potato and tomato late blight. However, different triterpenoids could have contrasting functions on plant resistance against P. infestans. In this study, we targeted the key biosynthetic gene of all plant triterpenoids, SQUALENE SYNTHASE (SQS), to examine the function of this gene in plant-P. infestans interactions. A post-inoculation, time-course gene expression analysis revealed that SQS expression was induced in Nicotiana benthamiana but was transiently suppressed in Solanum lycopersicum. Consistent with the host-specific changes in SQS expression, concentrations of major triterpenoid compounds were only induced in S. lycopersicum. A stable overexpression of SQS in N. benthamiana reduced plant resistance against P. infestans and induced the hyperaccumulation of stigmasterol. A comparative transcriptomics analysis of the transgenic lines showed that diverse plant physiological processes were influenced by SQS overexpression, suggesting that phytosterol content regulation may not be the sole mechanism through which SQS promotes plant susceptibility towards P. infestans. This study provides experimental evidence for the host-specific transcriptional regulation and function of SQS in plant interactions with P. infestans, offering a novel perspective in examining the quantitative disease resistance against late blight.

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