A highly sensitive aptasensor based on 3D-rGO/AuNPs for Hg2+ determination using HCR amplification strategy triggered by T-Hg2+-T

文献类型： 外文期刊

第一作者： Shao, Yangyang

作者： Shao, Yangyang;Dong, Yanjie;Fan, Lixia;Bin, Liu;Wang, Lei;Zhao, Shancang;Shao, Yangyang;Dong, Yanjie;Fan, Lixia;Bin, Liu;Wang, Lei;Zhao, Shancang;Shao, Yangyang;Li, Dapeng;Xu, Weidong

作者机构：

关键词： Aptasensor; Three-dimensional reduced graphene oxide; gold nanoparticles; Hybridization chain reaction; T-Hg2+-T; Toluidine blue

期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.304； 五年影响因子：4.723 ）

ISSN： 0026-265X

年卷期： 2022 年 177 卷

页码：

收录情况： SCI

摘要： In this paper, we developed an electrochemical aptasensor with high sensitivity based on the stable T-Hg2+-T structure for Hg2+ determination. The three-dimensional reduced graphene oxide-modified gold nanoparticles (3D-rGO/AuNPs) was served as support material, which offered more immobilization sites for thiolated probe (P1). In the presence of Hg2+, P1 could interact with another probe (P2) by forming T-Hg2+-T structure. Further, the partial sequence of P2 that did not interact with P1 was employed as an initiator sequence, which resulted in the formation of the extended double-stranded DNA through the hybridization chain reaction (HCR) between two harpin DNA strands (HP1 and HP2). Toluidine blue (TB) was used to interact with the double-stranded DNA and bring in effective electrochemical responses. Under the optimal experimental conditions, the aptasensor exhibited pronounced electrochemical signals towards Hg2+ ranging from 10 fM to 50 nM, with a low detection limit of 3.3 fM. Besides, the aptasensor showed excellent performance in water samples for Hg2+ determination.

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