Preparation of functional oils rich in diverse medium and long-chain triacylglycerols based on a broadly applicable solvent-free enzymatic strategy

文献类型： 外文期刊

第一作者： Lai, Yundong

作者： Lai, Yundong;Liu, Tieliang;Wan, Chuyun;Zhang, Yi;Zhang, Yufei;Zheng, Mingming;Lai, Yundong;Li, Dongming

作者机构：

关键词： Structured triacylglycerol; Enzymatic interesterification; Essential fatty acids; Hydrophobic porous materials; Immobilization enzyme; Candida sp; lipase (CSL); Nervonic acid

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：8.1； 五年影响因子：7.7 ）

ISSN： 0963-9969

年卷期： 2023 年 164 卷

页码：

收录情况： SCI

摘要： To address the problems of long reaction times and limited range of adaptation in enzymatic synthesis medium and long-chain triacylglycerols (MLCTs), a broadly applicable solvent-free enzymatic interesterification strategy was proposed. Candida sp. lipase (CSL) was immobilized on hydrophobic hollow mesoporous silica spheres (HHSS) to construct a biocatalyst designated as CSL@HHSS with a 15.3 % immobilization yield and a loading amount of 94.0 mg/g. The expressed activity and the specific activity were 20.14 U/g and 173.62 U/g, which were 4.6 and 5.6 times higher than that of free CSL, respectively. This biocatalyst demonstrated higher activity, wider applicability, and excellent reusability. Linseed oil, sunflower oil, perilla seed oil, algal oil, and malania oleifera oil were applied as substrates to produce MLCTs with medium-chain triacylglycerols (MCT) catalyzed by CSL@HHSS through interesterification in yields ranging from 69.6 % to 78.0 % within 20 min. Specific fatty acids, including linolenic acid, oleic acid, DHA, and nervonic acid (the first reported), were introduced into MLCT's skeleton, respectively. The structures were finely analyzed and identified by GC and UPLC-MS. The catalytic efficiency value of CSL@HHSS in catalyzing interesterification between linseed oil and MCT (70 celcius, 20 min, lipase 6 wt%) is 0.86 g/g center dot min, which is the highest ever reported. This paper presents an effective and sustainable strategy for functional MLCTs production.

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