Involvement of Holotrichia parallela odorant-binding protein 3 in the localization of oviposition sites

文献类型： 外文期刊

第一作者： Li, Er-Tao

作者： Li, Er-Tao;Wu, Han-Jia;Qin, Jian-Hui;Luo, Jing;Li, Ke-Bin;Cao, Ya-Zhong;Zhang, Shuai;Yin, Jiao;Wu, Han-Jia;Peng, Yu

作者机构：

关键词： Holotrichia parallela; Odorant binding protein; Fluorescence competitive binding assay; RNA interference; Molecular docking

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2023 年 242 卷

页码：

收录情况： SCI

摘要： Organic fertilizers-derived volatiles attract Holotrichia parallela during oviposition. However, the mechanisms underlying the perception of oviposition cues in H. parallela remain unclear. Here, H. parallela odorant-binding protein 3 (HparOBP3) was identified as a key OBP. Bioinformatics analysis showed that HparOBP3 clustered together with Holotrichia oblita OBP8. HparOBP3 was mainly expressed in the antennae of both sexes. Recom-binant HparOBP3 exhibited distinct binding affinities towards 22 compounds released by organic fertilizers. After 48 h of RNA interference (RNAi), the expression of HparOBP3 in male and female antennae was decreased by 90.77 % and 82.30 %, respectively. In addition, silencing of HparOBP3 significantly reduced the electro-physiological responses and tropism of males to cis-3-hexen-1-ol, 1-hexanol, and (Z)-beta-ocimene as well as females to cis-3-hexen-1-ol, 1-hexanol, benzaldehyde, and (Z)-beta-ocimene. Molecular docking indicated that hydrophobic residues Leu-83, Leu-87, Phe-108, and Ile-120 of HparOBP3 were important amino acids for interacting with ligands. Mutation of the key residue, Leu-83, significantly diminished the binding ability of HparOBP3. Furthermore, acrylic plastic arena bioassays showed that the attraction and oviposition indexes of organic fer-tilizers to H. parallela were reduced by 55.78 % and 60.11 %, respectively, after silencing HparOBP3. These results suggest that HparOBP3 is essential in mediating the oviposition behavior of H. parallela.

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