Characterization of centromeric DNA of Gossypium anomalum reveals sequence-independent enrichment dynamics of centromeric repeats

文献类型： 外文期刊

第一作者： Ding, Wenjie

作者： Ding, Wenjie;Zhu, Yuanbin;Han, Jinlei;Zhang, Hui;Wang, Kai;Zhu, Yuanbin;Xu, Zhenzhen;Shen, Xinlian;Khurshid, Haris;Liu, Fang;Hasterok, Robert

作者机构：

关键词： Centromere; Centromeric retrotransposon; Adaptive evolution; Chromatin immunoprecipitation; Gossypium anomalum; CENH3

期刊名称：CHROMOSOME RESEARCH （ 影响因子：2.6； 五年影响因子：3.6 ）

ISSN： 0967-3849

年卷期： 2023 年 31 卷 2 期

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收录情况： SCI

摘要： Centromeres in eukaryotes are composed of highly repetitive DNAs, which evolve rapidly and are thought to achieve a favorable structure in mature centromeres. However, how the centromeric repeat evolves into an adaptive structure is largely unknown. We characterized the centromeric sequences of Gossypium anomalum through chromatin immunoprecipitation against CENH3 antibodies. We revealed that the G. anomalum centromeres contained only retrotransposon-like repeats but were depleted in long arrays of satellites. These retrotransposon-like centromeric repeats were present in the African-Asian and Australian lineage species, suggesting that they might have arisen in the common ancestor of these diploid species. Intriguingly, we observed a substantial increase and decrease in copy numbers among African-Asian and Australian lineages, respectively, for the retrotransposon-derived centromeric repeats without apparent structure or sequence variation in cotton. This result indicates that the sequence content is not a decisive aspect of the adaptive evolution of centromeric repeats or at least retrotransposon-like centromeric repeats. In addition, two active genes with potential roles in gametogenesis or flowering were identified in CENH3 nucleosome-binding regions. Our results provide new insights into the constitution of centromeric repetitive DNA and the adaptive evolution of centromeric repeats in plants.

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