Aloperine-Type Alkaloids with Antiviral and Antifungal Activities from the Seeds of Sophora alopecuroides L.

文献类型： 外文期刊

第一作者： Hu, Zhan-Xing

作者： Hu, Zhan-Xing;Zhang, Ji;Zhang, Tong;Tian, Cai-Yan;An, Qiao;Yi, Ping;Yuan, Chun-Mao;Hao, Xiao-Jiang;Hu, Zhan-Xing;Zhang, Ji;Zhang, Tong;Tian, Cai-Yan;An, Qiao;Yi, Ping;Yuan, Chun-Mao;Hao, Xiao-Jiang;Zhang, Zhong-Kai;Zhao, Li-Hua;Hao, Xiao-Jiang

作者机构：

关键词： Sophora alopecuroides L.; aloperine-typealkaloids; anti-TMV; antifungal activities

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 14 期

页码：

收录情况： SCI

摘要： As a continuous flow investigation of novel pesticides from natural quinolizidine alkaloids, the chemical compositions of the seeds of Sophora alopecuroides were thoroughly researched. Fifteen new aloperine-type alkaloids (1-15) as well as six known aloperine-type alkaloids (16-21) were obtained from the extract of S. alopecuroides. The structures of 1-21 were confirmed via HRESIMS, NMR, UV, IR, ECD calculations, and X-ray diffraction. The antiviral activities of 1-21 against tobacco mosaic virus (TMV) were detected following the improved method of half-leaf. Compared with ningnanmycin (protective: 69.7% and curative: 64.3%), 15 exhibited excellent protective (71.7%) and curative (64.6%) activities against TMV. Further biological studies illustrated that 15 significantly inhibited the transcription of the TMV-CP gene and increased the activities of polyphenol oxidase (PPO), peroxidase (POD), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL). The antifungal activities of 1-21 against Phytophythora capsica, Botrytis cinerea, Alternaria alternata, and Gibberella zeae were screened according to a mycelial inhibition test. Compound 13 displayed excellent antifungal activity against B. cinerea (EC50: 7.38 mu g/mL). Moreover, in vitro antifungal mechanism studies displayed that 13 causes accumulation of reactive oxygen species and finally leads to mycelia cell membrane damage and cell death in vitro.

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