Quantitative H-1 NMR for the Direct Quantification of Saikosaponins in Bupleurum chinense DC.

文献类型： 外文期刊

第一作者： Zhao, Hua

作者： Zhao, Hua;Wang, Xiaohui;Jiang, Yaling;Wang, Qiang;Zhao, Hua;Zhang, Yuli;Wang, Qiang;Huang, Xiaowan;Wang, Qiang;Ma, Huan;An, Li;Wu, Xujin

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关键词： Bupleurum chinense DC; quantitative H-1 NMR; molality; saikosaponin; quality evaluation

期刊名称：ANALYTICAL SCIENCES （ 影响因子：2.081； 五年影响因子：1.676 ）

ISSN： 0910-6340

年卷期： 2021 年 37 卷 10 期

页码：

收录情况： SCI

摘要： Saikosaponin a and saikosaponin d are used as chemical standards for the quality evaluation of Bupleurum chinense DC. by the high-performance liquid chromatography method in current Chinese Pharmacopoeia. However, other saikosaponins, such as saikosaponin c and saikosaponin b(2), also possess pharmaceutical activity, but are not used as chemical standards. In this study, a quantitative proton nuclear magnetic resonance (H-1 NMR) method was developed to determine the total mass percentage (mg/g) of SSa, SSb1, SSb2 and SSd in B. chinense DC., using the H-24 (delta(H) 0.71) signal. Furthermore, the molality (mol/kg) of type I saikosaponins (epoxy-ether structure) was also determined by quantitative H-1 NMR in the area of H-11 (delta(H) 5.95) for a more accurate quality evaluation. Validation of the method confirmed that it has acceptable selectivity, precision, stability, and repeatability. The results indicated that this method has the potential to be a reliable method for the quantification of saikosaponins in Bupleurum scorzonerifolium Willd., vinegar baked B. chinense DC. and B. scorzonerifolium Willd., Chaihu Koufuye (oral liquid of Chaihu).

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