Development and characterization of 60 novel EST-SSR markers in half-smooth tongue sole Cynoglossus semilaevis

文献类型： 外文期刊

第一作者： Sha, Z-X.

作者： Sha, Z-X.;Luo, X-H.;Liao, X-L.;Wang, Q-L.;Chen, S-L.;Wang, S-L.;Wang, S-L.

作者机构：

关键词： cross-species amplification;Cynoglossus semilaevis;EST-SSR;polymorphism

期刊名称：JOURNAL OF FISH BIOLOGY （ 影响因子：2.051； 五年影响因子：2.25 ）

ISSN： 0022-1112

年卷期： 2011 年 78 卷 1 期

页码：

收录情况： SCI

摘要： Sixty novel simple sequence repeat (SSR) markers were developed from expressed sequence tags (EST) of half-smooth tongue sole Cynoglossus semilaevis exploited in the laboratory. The number of alleles, observed and expected heterozygosity per locus ranged from two to 16, from 0.0833 to 1.0000 and from 0.0816 to 0.913, respectively. Of these SSRs, 20 had significant homology to known genes by BLASTx (basic local alignment search tool x) search. For cross-species amplification, there are 53 positive amplifications in Japanese flounder Paralichthys olivaceus with 12 polymorphic loci and 51 positive amplifications in Senegalese sole Solea senegalensis with 11 polymorphic loci. These new EST-SSR markers will be useful for genetic studies and genome mapping of C. semilaevis and its closely related fishes. (C) 2010 The Authors

分类号：

  • 相关文献

[1]Analysis of microsatellites in major crops assessed by computational and experimental approaches. Gao, LF,Tang, JF,Li, HW,Jia, JZ. 2003

[2]EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.). Li, Meng Jun,Xiao, Han,Wang, Xing Jun,Xia, Han,Zhao, Chuan Zhi,Bi, Yu Ping,Tang, Rong Hua. 2010

[3]Development of EST-SSR in foxtail millet (Setaria italica). Jia, Xiao-Ping,Shi, Yun-Su,Song, Yan-Cun,Wang, Guo-Ying,Wang, Tian-Yu,Li, Yu. 2007

[4]The development of EST-SSR markers in Lilium regale and their cross-amplification in related species. Yuan, Suxia,Ge, Liang,Liu, Chun,Ming, Jun.

[5]Genetic diversity in the germplasm of black pepper determined by EST-SSR markers. Wu, B. D.,Fan, R.,Hu, L. S.,Wu, H. S.,Hao, C. Y.,Wu, B. D.,Fan, R.,Hu, L. S.,Wu, H. S.,Hao, C. Y.. 2016

[6]MHC polymorphism and disease resistance to vibrio anguillarum in 8 families of half-smooth tongue sole (Cynoglossus semilaevis). Du, Min,Chen, Song-lin,Liu, Yang,Yang, Jing-feng,Du, Min,Du, Min,Liu, Yan-hong. 2011

[7]Development of New Microsatellite DNA Markers from Apostichopus japonicus and Their Cross-Species Application in Parastichopus parvimensis and Pathallus mollis. Liao, Meijie,Wang, Yingeng,Rong, Xiaojun,Zhang, Zheng,Li, Bin,Wang, Lan,Chen, Guiping. 2011

[8]Isolation of novel microsatellite markers from Paralichthys lethostigma (Paralichthyidae) and their cross-species application in Pleuronectiformes. Xu, Y. -J.,Liao, M. -J.,Wang, Y. -G.,Liu, Z. -C.,Qin, P.,Zhang, Z.,Rong, X. -J.,Liu, X. -Z.. 2013

[9]Isolation and characterization of 31 polymorphic microsatellite markers in barfin flounder (Verasper moseri) and the cross-species amplification in spotted halibut (Verasper variegatus). Ma, Hongyu,Chen, Songlin,Ma, Hongyu. 2009

[10]Polymorphic microsatellite markers in the false kelpfish Sebastiscus marmoratus: isolation, characterization, and cross-species amplification. Liu, H. B.,Liu, H. B.,Liu, S. F.,Ye, J. B.,Yuan, Y. J.,Zhuang, Z. M.,Yuan, Y. J.,Ding, S. X.. 2014

[11]Development of thirty-four novel polymorphic microsatellite markers in Coilia ectenes (Clupeiformes: Engraulidae) and cross-species amplification in two closely related taxa. Chen, Fang,Zeng, Liyan,Cheng, Qiqun,Chen, Fang. 2012

[12]Development of 20 Microsatellite Markers for Solenocera crassicornis and Their Cross-Species Application in Solenocera melantho. Liu, Shufang,Liu, Hongbo,Yuan, Yanjiao,Dai, Fangqun,Zhuang, Zhimeng,Liu, Hongbo,Lin, Lin,Yuan, Yanjiao. 2012

[13]Isolation and characterization of polymorphic microsatellite loci from an EST-library of red sea bream (Chrysophrys major) and cross-species amplification. Chen, SL,Liu, YG,Xu, MY,Li, J. 2005

[14]Development of thirty-five novel polymorphic microsatellite markers in Pseudosciaena polyactis (Perciformes:Sciaenidae) and cross-species amplification in closely related species, Pseudosciaena crocea. Chen, Wenming,Cheng, Qiqun,Chen, Wenming.

[15]Cross-species applicability of chicken microsatellite markers for investigation of genetic diversity in Indian duck (Anas platyrhynchos) populations. Mukesh,Sathyakumar, S.,Mukesh,Javed, Ruheena,Javed, Ruheena,Gaur, Uma,Han Jianlin. 2011

[16]Eight polymorphic microsatellite markers developed in the green leaf bug, Lygus lucorum Meyer-Dur (Hemiptera : Miridae). Liu, Yu-Di,Yang, Zhong-Cheng,Wu, Kong-Ming. 2007

[17]Thirteen microsatellite loci for Laodelphax striatellus and cross amplification in related taxa. Liu, Yudi,Hou, Maolin,Zhang, Baoqin. 2014

[18]Isolation and characterization of polymorphic microsatellite loci from a repeat-enriched genomic library of stone flounder (Kareius bicoloratus) and cross-species amplification. Tian, Yong-Sheng,Miao, Gui-Dong,Shao, Chang-Wei,Liao, Xiao-Lin,Chen, Song-Lin.

[19]Development of 12 polymorphic microsatellite markers in Coilia ectenes Jordan and Seale, 1905 (Clupeiformes: Engraulidae) and cross-species amplification in Coilia mystus Linnaeus, 1758. Ma, Chunyan,Cheng, Qiqun,Zhang, Qingyi.

[20]Isolation via enrichment and characterization of 14 dinucleotide microsatellite loci in one catfish, Glyptosternum maculatum and cross-amplification in other related taxa. Guo, Baoying,Xie, Congxin,Li, Hongjing,Shan, Lei,Wang, Huanling,Huo, Bin,Zhou, Qing,Zhang, Yan.

作者其他论文 更多>>

微信小程序