Various mobile genetic elements carrying optrA in Enterococcus faecium and Enterococcus faecalis isolates from swine within the same farm

文献类型： 外文期刊

第一作者： Xuan, Huiyong

作者： Xuan, Huiyong;Jia, Haiyan;Yao, Xiaohui;Wang, Shufeng;Wei, Jianchao;Li, Zongjie;Shao, Donghua;Liu, Ke;Qiu, Yafeng;Ma, Zhiyong;Li, Beibei;Xuan, Huiyong;Xia, Lining;Yao, Xiaohui;Wang, Shufeng;Schwarz, Stefan;Schwarz, Stefan;Li, Ruichao

作者机构：

期刊名称：JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY （ 影响因子：5.758； 五年影响因子：6.051 ）

ISSN： 0305-7453

年卷期：

页码：

收录情况： SCI

摘要： Objectives In this study, the distribution of the oxazolidinone/phenicol resistance gene optrA and the mobile genetic elements involved in its dissemination were analysed among enterococcal isolates from a farrow-to-finish swine farm. Methods Enterococcus faecium and Enterococcus faecalis isolates were obtained from all pig production stages in the farm. The optrA-carrying E. faecium and E. faecalis isolates were subjected to PFGE and antimicrobial susceptibility testing. Complete sequences of the genetically unrelated optrA-carrying E. faecium and E. faecalis isolates were determined using Illumina HiSeq and MinION platforms. Results The optrA gene was present in 12.2% (23/188) of the E. faecium and E. faecalis isolates, most of which originated from nursery and finishing stages. The 23 optrA-positive Enterococcus isolates represented 15 PFGE types. WGS of representative isolates of the 15 PFGE types showed that optrA was carried by diverse genetic elements either located in the chromosomal DNA or on plasmids. A novel optrA-bearing genetic element was identified on two distinct multi-resistance plasmids from E. faecium. Two new hybrid plasmids carrying several resistance genes were found in two E. faecalis isolates. pC25-1-like plasmids and chromosomally integrated Tn6674 and Tn6823-like transposons were prevalent in the remaining Enterococcus isolates. Conclusions The gene optrA was found in genetically unrelated E. faecium and E. faecalis isolates from the same farm. Analysis of the genetic contexts of optrA suggested that horizontal transfer including different plasmids and transposons played a key role in the dissemination of optrA in this farm.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序