DCL2-dependent regulation of sRNA biogenesis and translocation in soybean

文献类型： 外文期刊

第一作者： Kong, Xiangying

作者： Kong, Xiangying;Xu, Ruixin;Kong, Xiangying;Xu, Ruixin;Kong, Xiangying;Xu, Ruixin;Zhang, Yan;Qiu, Lijuan;Yan, Zhe;Lyu, Kaiwen;Lu, Dongdong;Liu, Yongqi;Li, Bosheng;Zhai, Jixian

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关键词： Soybean; DCL2-dependent small RNA; Graft; Mobile small RNAs

期刊名称：CROP JOURNAL （ 影响因子：5.6； 五年影响因子：6.0 ）

ISSN： 2095-5421

年卷期： 2025 年 13 卷 3 期

页码：

收录情况： SCI

摘要： Small RNAs (sRNAs) are essential for regulating plant growth and development, and they possess the notable ability to travel long distances within organisms to regulate target gene expression. Our study examined the dcl2 mutant, a key enzyme in sRNA biogenesis, to determine the role of the DCL2 protein in sRNA synthesis and to identify mobile sRNAs under DCL2 regulation. Through grafting experiments between dcl2 mutants and wild-type soybean plants, coupled with sRNA sequencing, we identified 14,105 sRNAs significantly affected by DCL2 and discovered 375 mobile sRNAs under its regulation. Degradome analysis provided deeper insights into the regulatory effects of these mobile sRNAs on their target genes, enabling us to understand their potential influences on plant development and stress responses. Leveraging the systemic movement of sRNAs from roots to shoots, we propose a novel strategy for manipulating gene expression in aboveground tissues. Overall, our research findings not only deepen our understanding of the complex regulatory networks involving mobile sRNAs regulated by DCL2, but also provide a new strategy for gene regulation, which could have a positive impact on agricultural biotechnology. (c) 2025 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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