a-Linolenic acid-regulated testosterone biosynthesis via activation of the JNK-SF-1 signaling pathway in primary rooster Leydig cells

文献类型： 外文期刊

第一作者： Zhao, Zhi-Xian

作者： Zhao, Zhi-Xian;Shang, Ming-Yu;Long, Cheng;Guo, Yong;Sheng, Xi-Hui;Wang, Xiang-Guo;Xing, Kai;Xiao, Long-Fei;Qi, Xiao-Long;Shang, Ming-Yu;Yao, Xue-Jun;Gao, Xiao-Bo

作者机构：

关键词： JNK-SF-1 signaling pathway; a-Linolenic acid; Leydig cell; Testosterone

期刊名称：THERIOGENOLOGY （ 影响因子：2.8； 五年影响因子：2.6 ）

ISSN： 0093-691X

年卷期： 2023 年 209 卷

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收录情况： SCI

摘要： As a functional fatty acid, a-linolenic acid (ALA) is essential in promoting animal testosterone biosynthesis. This study investigated the effects of ALA on testosterone biosynthesis and the possible mechanism underlying the signaling pathway in primary Leydig cells of the rooster. Methods: Primary rooster Leydig cells were treated with ALA (0, 20, 40, or 80 & mu;mol/L) or pretreated with a p38 inhibitor (50 & mu;mol/ L), a c-Jun NH2-terminal kinase (JNK) inhibitor (20 & mu;mol/L), or an extracellular signal-regulated kinase (ERK) inhibitor (20 & mu;mol/L) before ALA treatment. Testosterone content in the conditioned culture medium was detected using an enzyme-linked immunosorbent assay (ELISA). The expression of steroidogenic enzymes and JNK-SF-1 signaling pathway factors was detected using real-time fluorescence quantitative PCR (qRT-PCR). Results: Supplementation with ALA significantly increased testosterone secretion within culture media (P < 0.05), and the optimized dose was 40 & mu;mol/L. Compared with the control group, steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme (P450scc), and 30-hydroxysteroid dehydrogenase (313-HSD) mRNA expression significantly increased (P < 0.05) in the 40 & mu;mol/L ALA group; 17-hydroxylase/c17-20 lyase (P450c17) and p38 mRNA expressions were not significantly different in the 40 & mu;mol/L ALA group; ERK and JNK mRNA expressions were significantly upregulated (P < 0.05) in 40 & mu;mol/L ALA group. In the inhibitor group, testosterone levels were significantly downregulated (P < 0.05). Compared with the 40 & mu;mol/L ALA group, StAR, P450scc, and P450c17 mRNA expressions were significantly decreased (P < 0.05), and 313-HSD mRNA expression in the p38 inhibitor group did not change; StAR, P450scc, and 313-HSD mRNA expressions were significantly decreased (P < 0.05), and P450c17 mRNA expression in ERK inhibitor group did not change; StAR, P450scc, 313-HSD, and P450c17 mRNA expressions were significantly decreased (P < 0.05) in JNK inhibitor group. Additionally, the increased steroidogenic factor 1 (SF-1) gene expression levels induced by ALA were reversed when the cells were pre-incubated with JNK and ERK inhibitors. The levels in the JNK inhibitor group were significantly lower than those in the control group (P < 0.05). Conclusion: ALA may promote testosterone biosynthesis by activating the JNK-SF-1 signaling pathway to upregulate StAR, P450scc, 30-HSD, and P450c17 expression in primary rooster Leydig cells.& COPY; 2023 Published by Elsevier Inc.

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