MFS Transporter Bdorwp Does Not Affect Antennal Electrophysiology but Regulates Reproductive Behaviors in Bactrocera dorsalis

文献类型： 外文期刊

第一作者： Zhang, Yan

作者： Zhang, Yan;Wuyun, Qiqige;Wang, Qi;Luo, Zhicai;Zhang, Jie;Yan, Shanchun;Yuan, Jinxi;Liu, Wei;Wang, Guirong

作者机构：

关键词： Bactrocera dorsalis; selectablemarker gene; Bdorwp; CRISPR/Cas9; moleculartargets

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 45 期

页码：

收录情况： SCI

摘要： Developing behavioral modifying chemicals through molecular targets is a promising way to improve semiochemical-based technology for pest management. Identifying molecular targets that affect insect behavior largely relies on functional genetic techniques such as deletions, insertions, and substitutions. Selectable markers have thus been developed to increase the efficiency of screening for successful editing events. However, the effect of selectable markers on relevant phenotypic traits needs to be considered. In this study, we cloned the wp gene ofBactrocera dorsalis. Knocking out Bdorwp causes white pupae phenotypes. Reproductive behaviors in both males and females were strongly regulated by Bdorwp. Remarkably, Bdorwp did not affect the antennal electrophysiology response to 63 chemical components with various structures. It is recommended to indirectly apply Bdorwp as a selectable marker in functional gene research on behavioral modifying chemicals. Moreover, Bdorwp could also be a potential molecular target for developing new insecticides for tephritid species control.

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