Regulation of hierarchical carbon substrate utilization, nitrogen fixation, and root colonization by the Hfq/Crc/CrcZY genes in Pseudomonas stutzeri

文献类型： 外文期刊

第一作者： Lv, Fanyang

作者： Lv, Fanyang;Zhan, Yuhua;Lu, Wei;Ke, Xiubin;Shao, Yahui;Ma, Yiyuan;Zheng, Juan;Yang, Zhimin;Jiang, Shanshan;Shang, Liguo;Ma, Yao;Yan, Yongliang;Lin, Min;Cheng, Lei;Lin, Min;Elmerich, Claudine

作者机构：

期刊名称：ISCIENCE （ 影响因子：6.107； 五年影响因子：6.233 ）

ISSN：

年卷期： 2022 年 25 卷 12 期

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收录情况： SCI

摘要： Bacteria of the genus Pseudomonas consume preferred carbon substrates in nearly reverse order to that of enterobacteria, and this process is controlled by RNA-binding translational repressors and regulatory ncRNA antagonists. Howev-er, their roles in microbe-plant interactions and the underlying mechanisms remain uncertain. Here we show that root-associated diazotrophic Pseudomonas stutzeri A1501 preferentially catabolizes succinate, followed by the less favor-able substrate citrate, and ultimately glucose. Furthermore, the Hfq/Crc/CrcZY regulatory system orchestrates this preference and contributes to optimal nitro-genase activity and efficient root colonization. Hfq has a central role in this regu-latory network through different mechanisms of action, including repressing the translation of substrate-specific catabolic genes, activating the nitrogenase gene nifH posttranscriptionally, and exerting a positive effect on the transcription of an exopolysaccharide gene cluster. Our results illustrate an Hfq-mediated mech-anism linking carbon metabolism to nitrogen fixation and root colonization, which may confer rhizobacteria competitive advantages in rhizosphere environments.

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