PbbHLH155 enhances iron deficiency tolerance in pear by directly activating PbFRO2 and PbbHLH38

文献类型： 外文期刊

第一作者： Dong, Weiyu

作者： Dong, Weiyu;Liu, Lun;Sun, Yu;Xu, Xiaoqian;Guo, Guoling;Heng, Wei;Jia, Bing;Jiao, Huijun;Wei, Shuwei

作者机构：

关键词： PbbHLH155; Fe deficiency; Pear calli; FCR activity; Acidification activity

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.1； 五年影响因子：6.2 ）

ISSN： 0981-9428

年卷期： 2024 年 213 卷

页码：

收录情况： SCI

摘要： Iron (Fe) deficiency is a general stress for many horticulture crops, causing leaf chlorosis and stunted growth. The basic-helix-loop-helix (bHLH) transcription factor (TF) was reported to function in Fe absorption; however, the regulatory mechanism of bHLH genes on iron absorption remains largely unclear in pear. In this study, we found that PbbHLH155 was significantly induced by Fe deficiency. Overexpression of PbbHLH155 in Arabidopsis thaliana and pear calli significantly increases resistance to Fe deficiency. The PbbHLH155-overexpressed Arabidopsis lines exhibited greener leaf color, higher Fe content, stronger Fe chelate reductase (FCR) and root acidification activity. The PbbHLH155 knockout pear calli showed lower Fe content and weaker FCR activity. Interestingly, PbbHLH155 inhibited the expressions of PbFRO2 and PbbHLH38, which were positive regulators in Fe-deficiency responses (FDR). Furthermore, yeast one-hybrid (Y1H) and Dual-Luciferase Reporter (DLR) assays revealed that PbbHLH155 directly binds to the promoters of PbFRO2 and PbbHLH38, thus activating their expression. Overall, our results showed that PbbHLH155 directly promote the expression of PbFRO2 and PbbHLH38 to activate FCR activity for iron absorption. This study provided valuable information for pear breeding.

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