Construction of a genetic linkage map for silver carp (Hypophthalmichthys molitrix)

文献类型： 外文期刊

第一作者： Zhang, L.

作者： Zhang, L.;Yang, G.;Guo, S.;Wei, Q.;Zou, G.

作者机构：

关键词： amplified fragment length polymorphism;Hypophthalmichthys molitrix;linkage map;microsatellite;silver carp

期刊名称：ANIMAL GENETICS （ 影响因子：3.169； 五年影响因子：3.058 ）

ISSN： 0268-9146

年卷期： 2010 年 41 卷 5 期

页码：

收录情况： SCI

摘要： P>For silver carp (Hypophthalmichthys molitrix), a combined microsatellite (or simple sequence repeat) and amplified fragment length polymorphism (AFLP) sex average linkage map was constructed. A total of 483 markers (245 microsatellites and 238 AFLPs) were assigned to 33 linkage groups. The map spanned 1352.2 cM, covering 86.4% of the estimated genome size of silver carp. The maximum and average spaces between 420 loci were 21.5 cM and 3.2 cM, respectively. The length of linkage groups ranged from 3.6 cM to 98.5 cM with an average of 41.0 cM. The number of markers per group varied from 2 to 44 with an average of 14.6. The AFLP markers significantly improved the integrity of microsatellite-based linkage groups and increased the genome coverage and marker evenness. A genome-wide recombination suppression was observed in male. In an extreme case, six microsatellites co-segregated in male, but spanned a 45.1 cM region in female.

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