Genetic Characterization and Phylogenetic Analysis of Fasciola Species Isolated From Yaks on Qinghai-Tibet Plateau, China

文献类型： 外文期刊

第一作者： Gao, Xing

作者： Gao, Xing;Zhang, Zhao;Quan, Chuxian;Zhou, Shimeng;Li, Kewei;Li, Yan;Kulyar, Muhammad Fakhar-e-Alam;Li, Jiakui;Wang, Dongjing;Zeng, Jiangyong;Wang, Dongjing;Zeng, Jiangyong;Zhao, Suonan;Kong, Xiangying;Li, Jiakui

作者机构：

关键词： yaks; Fasciola intermediate; mitogenome; gene order; phylogenetic

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.471； 五年影响因子：3.821 ）

ISSN：

年卷期： 2022 年 9 卷

页码：

收录情况： SCI

摘要： The present study determined the complete mitochondrial DNA (mt DNA) sequence of Fasciola intermediate (isolated from yaks) based on gene content and genome organization. According to our findings, the genome of Fasciola intermediate was 13,960 bp in length, containing 2 ribosomal RNA (rRNA) genes, 12 protein-coding genes (PCGs), and 22 transfer RNA (tRNA) genes. The A+T content of genomes was 63.19%, with A (15.17%), C (9.31%), G (27.51%), and T as the nucleotide composition (48.02%). Meanwhile, the results showed negative AT-skew (-0.52) and positive GC-skew (0.494). The AT bias significantly affected both the codon usage pattern and amino acid composition of proteins. There were 2715 codons in all 12 protein-coding genes, excluding termination codons. Leu (16.72%) was the most often used amino acid, followed by Val (12.74%), Phe (10.90%), Ser (10.09%), and Gly (8.39%). A phylogenetic tree was built using Maximum-Likelihood (ML) through MEGA 11.0 software. The entire mt DNA sequence of Fasciola intermediate gave more genetic markers for investigating Trematoda population genetics, systematics, and phylogeography. Hence, for the first time, our study confirmed that yaks on the Qinghai-Tibet plateau have the infestation of Fasciola intermediate parasite.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序