The chemical composition, protective effect of Rheum officinale leaf juice and its mechanism against dextran sulfate sodium-induced ulcerative colitis

文献类型： 外文期刊

第一作者： Dai, Lixia

作者： Dai, Lixia;Miao, Xiaolou;Yang, Xiaorong;Zhang, Jiyu;Shang, Xiaofei;Cao, Xinyuan;Dai, Lixia;Zhang, Jiyu;Shang, Xiaofei

作者机构：

关键词： Rheum officinale leaf juice; Chemical ingredients; Ulcerative colitis; NF-kappa B/NLRP3; Gut microbiota

期刊名称：PHYTOMEDICINE （ 影响因子：7.9； 五年影响因子：6.8 ）

ISSN： 0944-7113

年卷期： 2024 年 129 卷

页码：

收录情况： SCI

摘要： Background: Rhubarb is widely distributed and cultivated worldwide, and its leaves presented antioxidant activity and could be used as food additive. However, the chemical ingredients, and protective effect of Rheum officinale leaf juice (JROL) on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) are still unclear. Purpose: This paper sought to the characterization and functional properties of JROL, and explore the underlying mechanism on UC mice. Methods: UPLC-ESI-Q-TOF/MS and other analytical instruments were employed to determine the chemical ingredients of JROL. After inducing UC model using 3% DSS, multiple biological methods were used to evaluate its protective effect and the potential mechanism. Results: JROL is rich in proximate compositions and minerals and has high nutritional value, and contains reducing sugars, polysaccharides and pectin. Fifteen compounds were identified using UPLC-ESI-Q-TOF/MS. Among them, rutin has the highest content (2.22 %) in UPLC analysis. JROL presented protective effect on DSS-induced UC, and alleviated morphological alterations and ultra-structural feature of tissue, and the polysaccharides and flavonoids may contribute to its protective effect. JROL inhibited NF-kappa B/NLRP3 signaling pathway to alleviate inflammatory response, oxidative stress and intestinal injury by decreasing the expression of p-p65, p-I kappa B alpha, NLRP3, ASC, etc.. Moreover, it up-regulated the expression of tight junction proteins, and rebalanced the disturbance of gut microbiota to regulate the inflammatory response. Finally, a correlation among the inflammatory response, NF-kappa B/NLRP3 pathway and gut microbiota was established. Moreover, JROL presented the safety in the acute toxicity test. Conclusion: JROL could be used as a potential new source for treating UC.

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