Molecular characterization and expression analysis of lipopolysaccharide and beta-1,3-glucan-binding protein (LGBP) from pearl oyster Pinctada fucata

文献类型： 外文期刊

第一作者： Zhang, Dianchang

作者： Zhang, Dianchang;Ma, Jianjun;Jiang, Jingjing;Qiu, Lihua;Zhu, Caiyan;Su, Tianfeng;Li, Youning;Wu, Kaichang;Jiang, Shigui;Ma, Jianjun

作者机构：

关键词： Pearl oyster;Pinctada fucata;Lipopolysaccharide and beta-1,3-glucan-binding protein;Pattern recognition protein

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN： 0301-4851

年卷期： 2010 年 37 卷 7 期

页码：

收录情况： SCI

摘要： The lipopolysaccharide and beta-1,3-glucan-binding protein (LGBP) plays an important function in the innate immune response of invertebrates as a pattern recognition receptor (PRR). Herein, we described the isolation and characterization of pearl oyster Pinctada fucata LGBP (designated as poLGBP). The poLGBP cDNA was 2,075 bp long and consisted of a 5'-untranslated region (UTR) of 18 bp, a 3'-UTR of 299 bp with one cytokine RNA instability motifs (ATTTA), and an open reading frame (ORF) of 1,758 bp encoding a polypeptide of 585 amino acids with an estimated molecular mass of 65.1 kDa and a theoretical isoelectric point of 5.80. Homology analysis of the deduced amino acid sequence of the poLGBP with other known LGBP sequences by MatGAT software revealed that the poLGBP shared 26.3-56.7% identity and 40.5-70.9% similarity to the other known LGBP sequences. SMART and alignment analysis revealed that the poLGBP possessed a potential polysaccharide-binding motif, a glucanase motif, a LPS-binding site, a beta-1,3-linkage of polysaccharide, a glycine-rich region, a threonine-rich region and two N-glycosylation sites. In healthy pearl oyster, the poLGBP mRNA was specifically expressed in digestive gland, and not detected in gill, adductor muscle, gonad, intestine, mantle and hemocytes. However, after bacteria stimulation, the expression of the poLGBP mRNA was significantly up-regulated in digestive gland and also weakly detected in haemocytes, gonad and intestine. After LPS stimulation, the poLGBP mRNA expression was significantly up-regulated at 8 and 12 h in digestive gland, and the expression level was 10.7-fold higher than the PBS group at 12 h. After bacteria stimulation, the expression level of the poLGBP mRNA was also significantly up-regulated in digestive gland and was 12.9-fold higher than the PBS group at 8 h. However, during the experiment, the poLGBP mRNA expression was not detected in gill after LPS or bacteria stimulation. The tissue-specific expression and the expression up-regulation after LPS or bacteria stimulation in digestive gland suggested that the poLGBP was an inducible acute-phase protein and might play an important function in digestion as digestive enzyme and pattern recognition receptor.

分类号：

  • 相关文献

[1]Molecular characterization and expression analysis of the I kappa B gene from pearl oyster Pinctada fucata. Xu, Xinping,Zhang, Dianchang,Jiang, Shigui,Qiu, Lihua,Su, Tianfeng,Wu, Kaichang,Li, Youning,Zhu, Caiyan,Zhang, Dianchang. 2009

[2]Molecular characterization and expression analysis of a putative LPS-induced TNF-alpha factor (LITAF) from pearl oyster Pinctada fucata. Zhang, Dianchang,Jiang, Jingjing,Jiang, Shigui,Ma, Jianjun,Su, Tianfeng,Qiu, Lihua,Zhu, Caiyan,Zhang, Dianchang,Jiang, Jingjing,Ma, Jianjun,Xu, Xinping,Xu, Xinping. 2009

[3]Isolation and screening of microsatellite markers from the pearl oyster, Pinctada fucata using FIASCO method. Yu, Da-Hui,Qu, Ni-Ni,Huang, Gui-Ju. 2015

[4]Molecular characterization and expression analysis of interferon-gamma-inducible lysosomal thiol reductase (GILT) gene from pearl oyster Pinctada fucata. Zhang, Dianchang,Pan, Dequan,Cui, Shuge,Su, Tianfeng,Qiu, Lihua,Zhu, Caiyan,Jiang, Shigui,Pan, Dequan,Cui, Shuge. 2010

[5]cDNA cloning and mRNA expression of a tandem-repeat galectin (PoGal2) from the pearl oyster, Pinctada fucata. Zhang, D. C.,Hu, Y. T.,Guo, H. Y.,Cui, S. G.,Su, T. F.,Jiang, S. G.,Hu, Y. T.,Guo, H. Y.,Jiang, S. G.. 2011

[6]A macrophage migration inhibitory factor like oxidoreductase from pearl oyster Pinctada fucata involved in innate immune responses. Cui, Shuge,Zhang, Dianchang,Jiang, Shigui,Hu, Yuting,Guo, Huayang,Chen, Mingqiang,Su, Tanfeng,Zhu, Caiyan,Cui, Shuge,Pu, Hanlin. 2011

[7]A multidomain galectin involved in innate immune response of pearl oyster Pinctada fucata. Zhang, Dianchang,Jiang, Shigui,Hu, Yuting,Cui, Shuge,Guo, Huayang,Wu, Kaichang,Li, Youning,Su, Tianfeng,Hu, Yuting,Guo, Huayang,Cui, Shuge.

[8]EXPRESSION, PURIFICATION, AND FUNCTIONAL ACTIVITY OF SHELL MATRIX PROTEIN PEARLIN FROM THE PEARL OYSTER PINCTADA FUCATA. Huang, Guiju,Bi, Xiaomin,Zhang, Bo,Qu, Tao,Liu, Baosuo,Fan, Sigang,Yu, Dahui,Bi, Xiaomin.

[9]A baseline study on lipid and fatty acid composition in the pearl oyster, Pinctada fucata. Shen, Ya-nan,Zhang, Bo,Jiang, Song,Liu, Bo-suo,Huang, Gui-ju,Yu, Da-hui,Shen, Ya-nan,Zhang, Bo,Jiang, Song,Liu, Bo-suo,Huang, Gui-ju,Yu, Da-hui,Shen, Ya-nan,Zhang, Bo,Jiang, Song,Liu, Bo-suo,Huang, Gui-ju,Yu, Da-hui,Shen, Ya-nan,Zhang, Dong-ling.

[10]Development and characterisation of polymorphic microsatellite markers in the pearl oyster Pteria penguin (Roding, 1798). Zhang, Nan,Ma, Zhenhua,Zhang, Dianchang,Guo, Huayang,Jiang, Shigui,Lin, Lin.

[11]Genetic characteristics of hybrid populations derived by crossing Chinese and Indian pearl oysters, Pinctada fucata, based on AFLP markers. Yu, Da Hui,Huang, Gui-ju,Guo, Yi-hui,Wang, Xiao-yu,Wang, Ai-min. 2009

[12]Characterization of 31 EST-derived microsatellite markers for the pearl oyster Pinctada martensii (Dunker). Shi, Yaohua,Wang, Yan,Hou, Zhanhui,Wang, Aimin,Shi, Yaohua,Guo, Ximing,Shi, Yaohua,Hong, Kui.

[13]Highly expressed EGFR in pearl sac may facilitate the pearl formation in the pearl oyster, Pinctada fucata. Zhu, Wenjie,Fan, Sigang,Huang, Guiju,Liu, Baosuo,Bi, Xiaomin,Yu, Dahui,Zhu, Wenjie,Fan, Sigang,Huang, Guiju,Liu, Baosuo,Bi, Xiaomin,Yu, Dahui,Zhu, Wenjie,Bi, Xiaomin,Zhang, Dongling.

[14]Genomic structure, characterization and expression analysis of a manganese superoxide dismutase from pearl oyster Pinctada fucata. Zhang, Dianchang,Cui, Shuge,Guo, Huayang,Jiang, Shigui,Zhang, Dianchang,Jiang, Shigui.

[15]Molecular characterization and mRNA expression of catalase from pearl oyster Pinctada fucata. Guo, Huayang,Zhang, Dianchang,Cui, Shuge,Chen, Mingqiang,Wu, Kaichang,Li, Youning,Su, Tianfeng,Jiang, Shigui,Guo, Huayang.

[16]Common pearl oysters in China, Japan, and Australia are conspecific: evidence from ITS sequences and AFLP. Yu, Da Hui,Jia, Xiaoping,Chu, Ka Hou. 2006

[17]Identification of twenty novel polymorphic microsatellite DNA markers from transcripts of the pearl oyster Pinctada fucata using next-generation sequencing approach. Fan, Sigang,Wang, Jingxuan,Huang, Guiju,Liu, Baosuo,Yu, Dahui,Wang, Jingxuan. 2015

[18]Transcriptome analysis of the immune reaction of the pearl oyster Pinctada fucata to xenograft from Pinctada maxima. Wei, Jinfen,Yu, Dahui,Wei, Jinfen,Fan, Sigang,Liu, Baosuo,Zhang, Bo,Su, Jiaqi,Wei, Jinfen.

[19]Identification and Differential Expression of Biomineralization Genes in the Mantle of Pearl Oyster Pinctada fucata. Li, Haimei,Yu, Dahui,Li, Haimei,Yu, Dahui,Li, Haimei,Zhang, Bo,Fan, Sigang,Liu, Baosuo,Su, Jiaqi,Yu, Dahui,Yu, Dahui,Yu, Dahui.

[20]A preliminary study for identification of candidate AFLP markers under artificial selection for shell color in pearl oyster Pinctada fucata. Zou, Keshu,Zhang, Dianchang,Guo, Huayang,Zhu, Caiyan,Li, Min,Jiang, Shigui,Zou, Keshu,Zhang, Dianchang,Li, Min,Jiang, Shigui.

作者其他论文 更多>>

微信小程序