Mechanistic insights into N2O emission mitigation by nitrification inhibitor dicyandiamide (DCD) in a tropical sandy soil after six years of manure amendment

文献类型： 外文期刊

第一作者： Fan, Changhua

作者： Fan, Changhua;Wang, Danfeng;Gao, Wenlong;Liu, Yuqin;Wu, Xiaolong;Liu, Huiran;Ning, Ziyu;Li, Qinfen;Chen, Miao;Fan, Changhua;Gao, Wenlong;Liu, Yuqin;Wu, Xiaolong;Liu, Huiran;Ning, Ziyu;Li, Qinfen;Chen, Miao;Fan, Changhua;Gao, Wenlong;Liu, Yuqin;Wu, Xiaolong;Liu, Huiran;Ning, Ziyu;Li, Qinfen;Chen, Miao;Wang, Danfeng;Duan, Pengpeng

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关键词： greenhouse gas; high-throughput sequencing; isotopocule mapping; tropical vegetable soil; organic amendment

期刊名称：PEDOSPHERE （ 影响因子：7.3； 五年影响因子：6.2 ）

ISSN： 1002-0160

年卷期： 2025 年 35 卷 3 期

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收录情况： SCI

摘要： Organic amendments (OM) can profoundly affect soil nitrous oxide (N2O) emissions via changing nitrogen (N) cycles. However, mechanistic insights into how nitrification inhibitors modulate the responses of soil N2O emissions to successive applications of OM are currently insufficient. In this study, we performed a laboratory experiment to examine N2O emissions from a tropical vegetable soil subjected to six years of chemical fertilization (CF) and chemical fertilization combined with manure application (CFM) and evaluate the mitigation effectiveness of nitrification inhibitor dicyandiamide (DCD) under each management regime. Isotopocule mapping showed that bacterial nitrification and/or fungal denitrification accounted for 77.4%-88.5% of total N2O production across treatments during the emission peak. The cumulative N2O emissions from the CFM-treated soil were nearly 8-fold of those from the CF-treated soil. The CFM treatment stimulated N2O production from bacterial nitrification and denitrification by increasing the abundance of genes linked to nitrifiers (ammonia-oxidizing bacterial (AOB) amoA and total comammox amoA) and denitrifiers (nirK, nirS, and qnorB), respectively. Importantly, DCD decreased cumulative N2O emissions by an average of 73.3%, with better mitigation performance observed in the CFM-treated soil than in the CF-treated soil due to stronger inhibited nitrification and increased abundance of the nosZ gene, and altered bacterial community composition. The 16S rRNA sequencing further revealed that adding DCD to the CFM-treated soil resulted in declines in the abundances of bacterial phylum Actinobacteria and Chloroflexi that positively affected N2O emissions; the opposite pattern prevailed for Gemmatimonadetes that negatively affected N2O emissions. This study highlights the potential of manure application, when coupled with nitrification inhibitors, to achieve the dual goals of enhancing soil fertility and reducing environmental risk associated with N2O emissions in tropical agricultural soils.

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