Molecular Cytogenetic Identification of the Wheat-Dasypyrum villosum T3DL.3V#3S Translocation Line with Resistance against Stripe Rust

文献类型： 外文期刊

第一作者： Zhang, Jie

作者： Zhang, Jie;Tang, Shuyao;Lang, Tao;Wang, Ying;Chen, Qian;Guo, Yuanlin;Xiao, Jun;Jiang, Yun;Zhang, Jie;Long, Hai;Deng, Guangbing;Xuan, Pu

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关键词： D. villosum; translocation line; stripe rust; molecular marker; ND-FISH

期刊名称：PLANTS-BASEL （ 影响因子：4.658； 五年影响因子：4.827 ）

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年卷期： 2022 年 11 卷 10 期

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收录情况： SCI

摘要： The annual species Dasypyrum villosum possesses several potentially valuable genes for the improvement of common wheat. Previously, we identified a new stripe rust-resistant line, the Chinese Spring (CS)-D. villosum 3V#3 (3D) substitution line (named CD-3), and mapped its potential rust resistance gene (designated as YrCD 3) on the 3V#3 chromosome originating from D. villosum. The objective of the present study was to further narrow down the YrCD 3 locus to a physical region and develop wheat-3V#3 introgression lines with strong stripe rust resistance. By treating CD-3 seeds with 60 Co y-irradiation, two CS-3V#3 translocation lines, T3V#3S.3DL and T3DS.3V#3L (termed 22-12 and 24-20, respectively), were identified from the M-4 generation through a combination of non-denaturing fluorescence in situ hybridization (ND-FISH) and functional molecular markers. Stripe rust resistance tests showed that the line 22-12 exhibited strong stripe rust resistance similarly to CD-3, whereas 24-20 was susceptible to stripe rust similarly to CS, indicating that YrCD-3 is located on the short arm of 3V#3. The line 22-12 can potentially be used for further wheat improvement. Additionally, to trace 3V#3 in the wheat genetic background, we produced 30 3V#3-specific sequence tag (EST) markers, among which, 11 markers could identify 3V#3S. These markers could be valuable in fine-mapping YrCD-3.

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