Effects of fermentation with Lactiplantibacillus plantarum NCU116 on the antihypertensive activity and protein structure of black sesame seed

文献类型： 外文期刊

第一作者： Du, Tonghao

作者： Du, Tonghao;Xu, Xiaoyan;Xiong, Shijin;Zhang, Linli;Xu, Yazhou;Zhao, Xueting;Huang, Tao;Xiao, Muyan;Xiong, Tao;Xie, Mingyong;Xiong, Tao;Xie, Mingyong;Huang, Jinqing;Huang, Tao;Xiao, Muyan

作者机构：

关键词： Black sesame seed; Fermentation; ACE inhibitory peptide; Protein structure; In silico screening; Molecular docking analysis

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2024 年 262 卷

页码：

收录情况： SCI

摘要： Effects of fermentation by Lactobacillus Plantarum NCU116 on the antihypertensive potential of black sesame seed (BSS) and structure characteristics of fermented black sesame seed protein (FBSSP) were investigated. Angiotensin-I-converting enzyme (ACE) inhibition and zinc chelating ability of fermented black sesame seed hydrolysate (FBSSH) reached the highest of 60.78 +/- 3.67 % and 2.93 +/- 0.04 mg/mL at 48 h and 60 h of fermentation, respectively. Additionally, the antioxidant activities of FBSSH and surface hydrophobicity of FBSSP were increased noticeably by fermentation. The alpha-helix and beta-rotation of FBSSP tended to decrease and increase, respectively, during fermentation. Correlation analysis indicated strong positive relationships between beta-turn and ACE inhibition activity as well as zinc chelating ability with correlation coefficients r of 0.8976 and 0.8932. Importantly, novel ACE inhibitory peptides LLLPYY (IC50 = 12.20 mu M) and ALIPSF (IC50 = 558.99 mu M) were screened from FBSSH at 48 h using in silico method. Both peptides showed high antioxidant activities in vitro. Molecular docking analysis demonstrated that the hydrogen bond connected with zinc ions of ACE mainly attributed to the potent ACE inhibitory activity of LLLPYY. The findings indicated that fermentation by Lactobacillus Plantarum NCU116 is an effective method to enhance the antihypertensive potential of BSS.

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