Molecular analysis of grass carp reovirus HZ08 genome segments 1-3 and 5-6

文献类型： 外文期刊

第一作者： Zhang, Chao

作者： Zhang, Chao;Wang, Qing;Shi, Cunbin;Zeng, Weiwei;Liu, Yongkui;Wu, Shuqin;Zhang, Chao;Liu, Yongkui

作者机构：

关键词： Grass carp reovirus;Aquareovirus;Sequencing;Phylogenetic analysis

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN： 0920-8569

年卷期： 2010 年 41 卷 1 期

页码：

收录情况： SCI

摘要： A new grass carp reovirus, assigned HZ08, was isolated from a diseased grass carp case during routine examination in Huzhou City, Zhejiang Province, China. The complete nucleotide sequences of genomic segments S1-S3 and S5-S6 were obtained and comprised 3927, 3870, 3753, 2229, and 2030 bp, respectively. Each segment contained a single open reading frame which encoded putative proteins of 143.6, 143.1, 135.9, 80.5, and 68.4 kDa, respectively. Conserved motifs 5' (GUAAUUUaEuro broken vertical bar UUCAUC) 3' were found at the ends of each segment. At the amino acid level, HZ08 S1-S3 and S5-S6 showed similarity to the corresponding segments of Aquareovirus. Further phylogenetic analysis using the amino acid sequences of the RNA-dependent RNA polymerases protein encoded by S2 revealed that HZ08 formed a cluster close to the aquareovirus, but was far from the other isolates, which indicated that HZ08 is likely to be a new member of Aquareovirus.

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