Construction of an Agrobacterium-mediated infectious cDNA clone of melon aphid-borne yellows virus

文献类型： 外文期刊

第一作者： Liu, Liming

作者： Liu, Liming;Ren, Qian;Peng, Bin;Kang, Baoshan;Wu, Huijie;Gu, Qinsheng

作者机构：

关键词： Melon aphid-borne yellows virus; Complete genome; Infectious cDNA clone; Vector transmission

期刊名称：VIRUS RESEARCH （ 影响因子：6.286； 五年影响因子：4.486 ）

ISSN： 0168-1702

年卷期： 2022 年 315 卷

页码：

收录情况： SCI

摘要： Melon aphid-borne yellows virus (MABYV), a member of the genus Polerovirus in the family Solemoviridae, has widely spread in recent years and cause yellowing disease on cucurbits. Here, we obtained the complete genome sequence of MABYV bottle guard (Lagenaria siceraria) isolate MABYV-KF, and constructed its infectious cDNA clone under the control of the cauliflower mosaic virus (CaMV) 355 promoter by Gibson assembly. The 5,677 nt of its genome shared more than 94.00% sequence identity with the two known MABYV isolates. The inoculation results showed that MABYV infectious cDNA clone could systemically infect bottle guard, cucumber and muskmelon plants, and cause typical yellowing symptom. The virus progeny from the infectious clone could be transmitted between bottle guard plants by aphid. Further analyses revealed that point mutations in the F-boxlike motif (Pro57) and C-terminal conserved sequence (Phe211) of PO cause low viral accumulations in systematic leaves and failed to induce symptom. The infectious clone will be potentially a tool in the investigation of viral pathogenesis, virus-virus interaction and virus-host/-vector interactions.

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