Tauroursodeoxycholic acid (TUDCA) improves intestinal barrier function associated with TGR5-MLCK pathway and the alteration of serum metabolites and gut bacteria in weaned piglets

文献类型： 外文期刊

第一作者： Song, Min

作者： Song, Min;Zhang, Fenglin;Fu, Yiming;Yi, Xin;Feng, Shengchun;Yang, Qiang;Zhu, Canjun;Zhu, Xiaotong;Wang, Lina;Gao, Ping;Shu, Gang;Jiang, Qingyan;Wang, Songbo;Song, Min;Zhang, Fenglin;Fu, Yiming;Yi, Xin;Feng, Shengchun;Yang, Qiang;Zhu, Canjun;Zhu, Xiaotong;Wang, Lina;Gao, Ping;Shu, Gang;Jiang, Qingyan;Wang, Songbo;Song, Min;Liu, Zhichang;Deng, Dun;Yu, Miao;Ma, Xianyong;Ma, Xianyong

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关键词： Gut bacteria; Intestinal barrier function; Serum metabolites; Tauroursodeoxycholic acid (TUDCA); TGR5-MLCK pathway; Weaned piglets

期刊名称：JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY （ 影响因子：6.175； 五年影响因子：6.853 ）

ISSN： 1674-9782

年卷期： 2022 年 13 卷 1 期

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收录情况： SCI

摘要： Background Tauroursodeoxycholic acid (TUDCA), a hydrophilic bile acid, is the main medicinal component of bear bile and is commonly used to treat a variety of hepatobiliary diseases. Meanwhile, TUDCA has been shown to modulate the intestinal barrier function and alleviate DSS-induced colitis in mice. However, the effect of TUDCA on the intestinal barrier of weaned piglets remains largely unclear. Methods The weaned piglets and porcine IPEC-J2 intestinal epithelial cells were used to investigate the effects of TUDCA on intestinal barrier function in weaned piglets and explore the possible underlying mechanisms. In vivo, 72 healthy weaned piglets were randomly allocated into 2 groups according to their gender and body weight, and piglets were fed the basal diet with 0 (control, CON) and 200 mg/kg TUDCA for 30 d, respectively. Three female and three male piglets reflecting the average bodyweight were slaughtered in each group and samples were collected. In vitro, IPEC-J2 cells were subjected to 100 mu mol/L TUDCA to explore the possible underlying mechanisms. Results Our results demonstrated that dietary TUDCA supplementation significantly reduced the diarrhea incidence of weaned piglets, possibly attributing to the TUDCA-enhanced intestinal barrier function and immunity. In addition, TUDCA supplementation altered serum metabolites and the relative abundance of certain gut bacteria, which might contribute to the improved intestinal barrier function. Furthermore, the in-vitro results showed that TUDCA improved the E. coli-induced epithelial barrier impairment of IPEC-J2 cells and increased Takeda G-coupled protein receptor 5 (TGR5) protein expression. However, knockdown of TGR5 and inhibition of myosin light chain kinase (MLCK) pathway abolished the TUDCA-improved epithelial barrier impairment in E. coli-treated IPEC-J2 cells, indicating the involvement of TGR5-MLCK in this process. Conclusions These findings showed that TUDCA improved intestinal barrier function associated with TGR5-MLCK pathway and the alteration of serum metabolites and gut bacteria in weaned piglets, suggesting the potential application of TUDCA in improving gut health in piglet production.

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