Simultaneous determination of 58 glucocorticoid residues in milk by ultra-high performance liquid chromatography-tandem mass spectrometry

文献类型： 外文期刊

第一作者： Guo, Can

作者： Guo, Can;Tan, Sijia;Chen, Gang;Wu, Cuiling;Zhang, Zihao;Chen, Sumeng;Guo, Can

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关键词： Glucocorticoid; Milk; Liquid chromatography -tandem mass; spectrometry

期刊名称：JOURNAL OF CHROMATOGRAPHY A （ 影响因子：4.1； 五年影响因子：3.7 ）

ISSN： 0021-9673

年卷期： 2024 年 1719 卷

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收录情况： SCI

摘要： Abuse of glucocorticoid veterinary drugs in dairy industry can potentially threat milk safety and consequently influence human health. Here a reliable method for determination of 58 glucocorticoid drug residues in milk was established by combining solid phase extraction with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The analytes were extracted with acetonitrile and cleanup with EMRLipid lipid removal column. The analytes were chromatographically separated using Poroshell EC-C18 column and acquired by electrospray ionization with multiple-reaction monitoring (MRM) mode. The limit of quantification (S/N >= 10) ranged from 0.2 to 2.0 mu g/kg and the limit of detection (S/N >= 3) ranged from 0.1 to 1.0 mu g/ kg. Average recoveries were from 71% to 113%, the relative standard deviations (RSDs) were less than 15%, and the correlation coefficients (R2) of calibration curves exceeded 0.99. The method was applied to detect twenty milk products obtained from local supermarkets including ten pasteurized milk and ten UHT milk. Two endogenous glucocorticoids, i.e. hydrocortisone and cortisone were detected but not exceed the maximum residue limits (MRLs).

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