Molecular Characteristics and Potent Immunomodulatory Activity of Fasciola hepatica Cystatin

文献类型： 外文期刊

第一作者： Zhang, Kai

作者： Zhang, Kai;Liu, Yucheng;Zhang, Guowu;Wang, Xifeng;Li, Zhiyuan;Shang, Yunxia;Ning, Chengcheng;Ji, Chunhui;Xia, Xianzhu;Qiao, Jun;Meng, Qingling;Cai, Xuepeng

作者机构：

关键词： Fasciola hepatica; cystatin; mouse macrophage; immunomodulation; Ethical approval

期刊名称：KOREAN JOURNAL OF PARASITOLOGY （ 影响因子：1.776； 五年影响因子：1.773 ）

ISSN： 0023-4001

年卷期： 2022 年 60 卷 2 期

页码：

收录情况： SCI

摘要： Cystatin, a cysteine protease inhibitor found in many parasites, plays important roles in immune evasion. This study analyzed the molecular characteristics of a cystatin from Fasciola hepatica (FhCystatin) and expressed recombinant FhCystatin (rFhcystatin) to investigate the immune modulatory effects on lipopolysaccharide-induced proliferation, migration, cytokine secretion, nitric oxide (NO) production, and apoptosis in mouse macrophages. The FhCystatin gene encoded 116 amino acids and contained a conserved cystatin-like domain. rFhCystatin significantly inhibited the activity of cathepsin B. rFhCystatin bound to the surface of mouse RAW264.7 cells, significantly inhibited cell proliferation and promoted apoptosis. Moreover, rFhCystatin inhibited the expression of cellular nitric oxide, interleukin-6, and tumor necrosis factor-alpha, and promoted the expression of transforming growth factor-beta and interleukin-10. These results showed that FhCystatin played an important role in regulating the activity of mouse macrophages. Our findings provide new insights into mechanisms underlying the immune evasion and contribute to the exploration of potential targets for the development of new drug to control F. hepatica infection.

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