Inhibition of porcine transmissible gastroenteritis virus (TGEV) replication in mini-pigs by shRNA

文献类型： 外文期刊

第一作者： Zhou, Junfang

作者： Zhou, Junfang;Huang, Fen;Hua, Xiuguo;Cui, Li;Zhang, Wen;Shen, Yan;Yan, Yijia;Chen, Piren;Mou, Jing;Lan, Daoliang;Yang, Zhibiao;Zhou, Junfang;Ding, Dezhong;Chen, Qi

作者机构：

关键词： TGEV;shRNA;Mini-pig

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2010 年 149 卷 1 期

页码：

收录情况： SCI

摘要： Transmissible gastroenteritis virus (TGEV) is the causative agent of porcine transmissible gastroenteritis (TGE), characterized by high mortality and severely retarded growth in piglets that dramatically affects the porcine industry. Previously, we have identified two shRNA-expressing plasmids pEGFP-U6/P1 and pEGFP-U6/P2 that target RNA-dependent RNA polymerase (RdRP) gene of TGEV with more than 95% of virus inhibition in vitro. In this study, inhibition of the TGEV replication by pEGFP-U6/P1 and pEGFP-U6/P2 was tested in mini-pigs. SPF mini-pigs at 25 days old were injected with the shRNA-expressing plasmids and then infected with TGEV. The results from the analyses of clinical signs, histopathology, indirect immunofluorescence (IIF) and RT-PCR show that the two shRNA-expressing plasmids could significantly decrease the quantity of TGEV in different organs and protect mini-pigs from TGEV infection. These findings illustrate the prospect for TGEV-specific shRNAs to be new anti-TGEV agents. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.

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