Identification of the S-genotypes of several sweet cherry (Prunus avium L.) cultivars by AS-PCR and pollination

文献类型： 外文期刊

第一作者： Wang, Hongxia

作者： Wang, Hongxia;Su, Huairui;Naihaoye;Wang, Hongxia;Zhang, KaiChun;Naihaoye

作者机构：

关键词： Self-incompatibility;Prunus avium;S-RNase;AS-PCR;pollination test

期刊名称：AFRICAN JOURNAL OF AGRICULTURAL RESEARCH （ 影响因子：0.263； 五年影响因子：0.203 ）

ISSN： 1991-637X

年卷期： 2010 年 5 卷 3 期

页码：

收录情况： SCI

摘要： Sweet cherry cultivars display a self-incompatibility system that restricts self-fertilization and fertilization between cultivars bearing identical S-alleles. PCR-based S-allele typing system for sweet cherry cultivars has been recently developed. It has been reported that all known self-incompatibility ( S) alleles of sweet cherry were more than 16 based on the AS-PCR analysis. In this work, two sets of AS-PCR primers that were designed based on conserved domains of cDNA sequences of S-RNases has been used to characterize the S-genotype of 38 sweet cherry cultivars, including 15 cultivars whose S-genotype had not been previously described. Pollination test was also performed to validate the PCR results, and the consistent results were got. A wide variation in the frequency of S-alleles in the Sweet cherry germplasm was observed. S(3) was the most common in the cultivars evaluated, and S(7), S(10)-S(16), S(23)-S(25) as rare allele was not found in China geographical areas in our study.

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