Cloning, Characterization of Landes Goose C/EBP beta gene and its mRNA Expression response to Dietary Betaine Supplementation in Fatty Liver

文献类型： 外文期刊

第一作者： Zhang, Xiang

作者： Zhang, Xiang;Li, Qifa;Su, Shengyan;Yu, Shali;Zhou, Yang;Xie, Zhuang;Su, Shengyan;Su, Shengyan

作者机构：

关键词： Overfeedings;Landes geese;C/EBP beta gene;Betaine

期刊名称：2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010)

ISSN： 2151-7614

年卷期： 2010 年

页码：

收录情况： SCI

摘要： The protein synthesised from the C/EBP beta gene interacts specifically with a C/EBP-recognition sequence and acts as a transcriptional activator. It plays a key role in lipometabolism and therefore is considered as a candidate gene for goose fat liver forming. In order to get the sequence, four pairs specificity primers were designed for amplification of C/EBP beta gene in Landes geese. The fragments of C/EBP beta gene in geese were obtained by PCR amplification and splicing. The length of the complete DNA sequence was 2130 bp (Genebank accession number GU068582), including the promoter regions and a 984 bp open reading frame coding 327 amino acid(aa). High percentages of G and C nucleotides were found in the coding region and 5' flanking region comprising a CpG island. The corresponding mRNA is present at high levels in the liver and adipose tissue compared to others. This indicated that it was closely related with lipid metabolism. In liver, the expression of C/EBP beta mRNA was higher in geese overfed than that of control group (P< 0.01). Compared with the overfed groups, the geese that were fed the betaine supplemented diet showed the decreased expression on the transcriptive level in the livers.

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