Comparison of chromosome preparation methods for the different developmental stages of the half-smooth tongue sole, Cynoglossus semilaevis

文献类型： 外文期刊

第一作者： Shao, Chang-Wei

作者： Shao, Chang-Wei;Wu, Peng-Fei;Wang, Xian-Li;Tian, Yong-Sheng;Chen, Song-Lin

作者机构：

关键词： Chromosome preparation;Cynoglossus semilaevis;Developmental stages;Half-smooth tongue sole;Sex chromosome

期刊名称：MICRON （ 影响因子：2.251； 五年影响因子：1.969 ）

ISSN： 0968-4328

年卷期： 2010 年 41 卷 1 期

页码：

收录情况： SCI

摘要： Here we present a comparison of the different chromosome preparation methods, including the method of the single embryo or larva, the method of juvenile-swimming and the method of phytohemagglutinin injection, used for the different developmental stages of the half-smooth tongue sole, Cynoglossus semilaevis. The mean index of mitosis for the three methods was 0.79%, 0.09% and 0.15%, respectively. From the well-spread metaphase chromosomes obtained, it was determined that C semilaevis has a diploid chromosome number of 42 and heterotypic sex chromosomes. Also, the metaphase chromosomes produced could be used for karyotype preparation and banding studies. The three methods are also successfully used for chromosome preparation in Japanese flounder (Paralichthys olivaceus) and summer flounder (Paralichthys dentatus). (C) 2009 Elsevier Ltd. All rights reserved.

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