Engineering and Optimization of the Salidroside Production in Tobacco Cells by Reconstructed Biosynthetic Genes from Rhodiola rosea

文献类型： 外文期刊

第一作者： Sun, Huapeng

作者： Sun, Huapeng;Tang, Xin;Zhang, Yingying;Fu, Xiaona;Wang, Rongxiang;Shahzaib, Muhammad;Qiao, Fei

作者机构：

关键词： salidroside; reconstruction; heterologous production; tobacco cells; endogenous l-tyrosine

期刊名称：ACS SYNTHETIC BIOLOGY （ 影响因子：3.9； 五年影响因子：4.3 ）

ISSN： 2161-5063

年卷期： 2025 年 14 卷 7 期

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收录情况： SCI

摘要： Salidroside, a tyrosine-derived bioactive product, originates from the Rhodiola genus and has various medicinal properties. However, the surge in global demand requires exploration of more efficient and sustainable approaches to produce salidroside. This study reconstructed three functional enzymes in tobacco cells to produce salidroside using endogenous l-tyrosine. Under the optimized two-stage protocol (1% sucrose in the production medium; 11 days of culture), salidroside accumulation reached a maximum of 377.53 mu g per gram of HHU cells in fresh weight. Neither precursor feeding (100 mg/L shikimate) nor various elicitors (e.g., MeJA, SA, and ABA) provided any further enhancement. Comparative transcriptomic analysis revealed that two DAHPS transcripts (107797958 and 107817203) were upregulated more than 2-fold, indicating that the reconstructed pathway boosts endogenous l-tyrosine flux, and elicitation and precursor feeding strategies could not enhance salidroside accumulation. Meanwhile, comparative transcriptomic analysis revealed that the reconstructed pathway could upregulate crucial gene expression in endogenous l-tyrosine biosynthesis. This study evaluates tobacco cells as biofactories to produce salidroside and lays the foundation for the biosynthesis engineering of other heterologous metabolites.

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