文献类型： 外文期刊

第一作者： Dong Xuewei

作者： Dong Xuewei;Li Youzhi;He Qingfang;Peng Zhenying;Yu Jinhui;Bian Fei;Bi Yuping;He Qingfang

作者机构：

关键词： Synechococcus sp PCC7002; Synechocystis sp PCC6803; Delta 15 fatty acid desaturase; Delta 6 fatty acid desaturase; polyunsaturated fatty acids

期刊名称：CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.068； 五年影响因子：0.983 ）

ISSN： 0254-4059

年卷期： 2016 年 34 卷 4 期

页码：

收录情况： SCI

摘要： Genetic modification is useful for improving the nutritional qualities of cyanobacteria. To increase the total unsaturated fatty acid content, along with the ratio of omega-3/omega-6 fatty acids, genetic engineering can be used to modify fatty acid metabolism. Synechococcus sp. PCC7002, a fast-growing cyanobacterium, does not contain a Delta 6 desaturase gene and is therefore unable to synthesize gamma-linolenic acid (GLA) and stearidonic acid (SDA), which are important in human health. In this work, we constructed recombinant vectors Syd6D, Syd15D and Syd6Dd15D to express the Delta 15 desaturase and Delta 6 desaturase genes from Synechocystis PCC6803 in Synechococcus sp. PCC7002, with the aim of expressing polyunsaturated fatty acids. Overexpression of the Delta 15 desaturase gene in Synechococcus resulted in 5.4 times greater accumulation of alpha-linolenic acid compared with the wild-type while Delta 6 desaturase gene expression produced both GLA and SDA. Co-expression of the two genes resulted in low-level accumulation of GLA but much larger amounts of SDA, accounting for as much to 11.64% of the total fatty acid content.

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