Salipaludibacillus daqingensis sp. nov., a moderately halophilic bacterium isolated from an oilfield

文献类型： 外文期刊

第一作者： Guo, Liwei

作者： Guo, Liwei;Liu, Jiading;Hou, Qiang;Zhao, Baisuo;Wang, Haisheng;Zhang, Xiaoxia

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关键词： Salipaludibacillus daqingensis sp. nov.; alkaliphile; moderately halophilic; oil field; Salipaludibacillus

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.8； 五年影响因子：2.6 ）

ISSN： 1466-5026

年卷期： 2023 年 73 卷 11 期

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收录情况： SCI

摘要： A novel alkaliphilic, Gram-stain-positive, moderately halophilic, rod-shaped, endospore-forming, motile, facultatively anaerobic bacterium (DQ-9(T)) was isolated from a sediment sample collected from Daqing oilfield in China, and characterized by a polyphasic taxonomic approach. Strain DQ-9(T) formed yellow pigment and grew occurred at salinities of 1-12% (w/v) NaCl (optimum, 8 %) and at 10-40 degrees C (optimum, 30-35 degrees C), at pH 7.5-10.5 (optimum, pH 9.0-9.5). It was catalase-positive, but oxidase-negative. Based on the analysis of 16S rRNA gene sequences, DQ-9(T) was classified into the genus Salipaludibacillus and exhibited the highest similarities (98.37 %) to Salipaludibacillus neizhouensis JSM 071004(T). Digital DNA-DNA hybridization and average nucleotide identity values between strain DQ-9(T) and the most closely related strain, S. neizhouensis DSM 19794(T), were determined to be 72.0 and 21.6%, respectively. The polar lipids were constituted by diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids (>5%) comprised anteiso-C-15:0, anteiso-C-17:0, iso-C-17:0, iso-C-15:0 and C-16:0. The cell-wall peptidoglycan contained meso-diaminopimelic acid, and menaquinone-7 was identified as the primary respiratory quinone. The DNA G+C content was 37.5 mol%. Through chemotaxonomic, physiological, and biochemical characterization, strain DQ-9(T) could be clearly distinguished from the closest Salipaludibacillus species. Based on provided data, strain DQ-9(T) is proposed to represent a novel species, Salipaludibacillus daqingensis sp. nov., within the genus Salipaludibacillus. The type strain is DQ-9(T) (=ACCC 60415(T)=KCTC 33936(T)).

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