A sensitive fluoroimmunoassay for quantitative detection of imidacloprid based on quantum dot-streptavidin conjugate
文献类型： 外文期刊
第一作者： Zhang, Binbin
作者： Zhang, Binbin;Li, Shi;Dong, Fengshou;Xu, Jun;Wu, Xiaohu;Zheng, Yongquan;Pan, Xinglu
作者机构：
关键词： Imidacloprid; Fluoroimmunoassay; Quantum dot-streptavidin conjugate; Rapid detection
期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.4 ）
ISSN： 0039-9140
年卷期： 2024 年 275 卷
页码：
收录情况： SCI
摘要： Imidacloprid (IMI), the most commonly used neonicotinoid, is widely present in both the environment and agroproducts due to extensive and prolonged application, posing potential risks to ecological security and human health. This study introduced a sensitive and rapid fluorescence-linked immunosorbent assay, employing Quantum Dot-Streptavidin conjugate (QDs-SA-FLISA), for efficient monitoring of IMI residues in agro-products. Under optimized conditions, the QDs-SA-FLISA exhibited a half-maximal inhibition concentration (IC50) of 1.70 ng/mL and a limit of detection (LOD, IC20) of 0.5 ng/mL. Investigation into the sensitivity enhancement effect of the QDs-SA revealed that the sensitivity (IC50) of the QDs-SA-FLISA was 7.3 times higher than that of ELISA. The recoveries and relative standard deviation (RSD) ranged from 81.7 to 118.1 % and 0.5-9.4 %, respectively, for IMI in brown rice, tomato and pear. There was no significant difference in IMI residues obtained between QDsSA-FLISA and UHPLC-MS/MS. Thus, the QDs-SA-FLISA represents a reliable approach for the quantitative determination of IMI in agro-products.
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