Identification of function modules in the co-expression protein-protein interaction network of Bombyx mori in response to Beauveria bassiana infection

文献类型： 外文期刊

第一作者： Lu, Dingding

作者： Lu, Dingding;Wang, Ya;Lu, Dingding;Wang, Zihe;Qin, Sheng;Qin, Sheng

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关键词： Bombyx mori; Beauveria bassiana; Co-expression protein-protein interaction network; network; Innate immunity

期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.4； 五年影响因子：3.0 ）

ISSN： 0022-2011

年卷期： 2024 年 207 卷

页码：

收录情况： SCI

摘要： Beauveria bassiana (B. bassiana) is a common fungal disease in sericulture. Previous research has primarily focused on investigating genes involved in innate immunity. However, the response of Bombyx mori (B. mori) to B. bassiana requires the coordination of other biological processes in addition to the immune system. We measured protein expression profile of B. mori after inoculating B. bassiana using iTRAQ technology in previous. Here we constructed a co-expression protein-protein interaction network of B. mori in response to B. bassiana infection. Subnetworks and modules were analyzed, and the functions of these modules were annotated. The results revealed the identification of numerous proteins associated with cellular immunity, including those involved in phagosomes, lysosomes, mTOR signaling, sugar metabolism, and the ubiquitin-proteasome pathway. Meanwhile, we observed that the pathways involved in protein synthesis were activated, including pyruvate and purine metabolism, RNA transport, ribosome, protein processing in endoplasmic reticulum, and protein export pathways, during B. bassiana infection. Based on this analysis, we selected six candidate genes (shock protein, ribosome, translocon, actin muscle-type A2, peptidoglycan recognition protein, and collagenase) that were found to be related to the response to B. bassiana. Further verification experiments demonstrated significant changes in their expression levels after inoculation with B. bassiana. These research findings provide new insights into the molecular mechanism of insect immune response to fungal infection.

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