Development of probiotic E. coli Nissle 1917 for beta-alanine production by using protein and metabolic engineering

文献类型： 外文期刊

第一作者： Hu, Shilong

作者： Hu, Shilong;Fei, Mingyue;Fu, Beibei;Yu, Mingjing;Yuan, Panhong;Sun, Dongchang;Tang, Biao;Yang, Hua

作者机构：

关键词： Escherichia coli Nissle 1917; beta-Alanine; Aspartate-alpha-decarboxylase; Genetic engineering; Fed-batch fermentation

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：5.0； 五年影响因子：5.2 ）

ISSN： 0175-7598

年卷期： 2023 年

页码：

收录情况： SCI

摘要： beta-alanine has been used in food and pharmaceutical industries. Although Escherichia coli Nissle 1917 (EcN) is generally considered safe and engineered as living therapeutics, engineering EcN for producing industrial metabolites has rarely been explored. Here, by protein and metabolic engineering, EcN was engineered for producing beta-alanine from glucose. First, an aspartate-alpha-decarboxylase variant ADC(K43Y) with improved activity was identified and over-expressed in EcN, promoting the titer of beta-alanine from an undetectable level to 0.46 g/L. Second, directing the metabolic flux towards L-aspartate increased the titer of beta-alanine to 0.92 g/L. Third, the yield of beta-alanine was elevated to 1.19 g/L by blocking conversion of phosphoenolpyruvate to pyruvate, and further increased to 2.14 g/L through optimizing culture medium. Finally, the engineered EcN produced 11.9 g/L beta-alanine in fed-batch fermentation. Our work not only shows the potential of EcN as a valuable industrial platform, but also facilitates production of beta-alanine via fermentation.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序